Lallement Claire, Goldring William P D, Jelsbak Lotte
Department of Sciences and Environment, Roskilde University, Roskilde, Denmark.
Arch Microbiol. 2023 Mar 16;205(4):117. doi: 10.1007/s00203-023-03450-x.
Bacterial intercellular signaling mediated by small molecules, also called autoinducers (AIs), enables synchronized behavior in response to environmental conditions, and in many bacterial pathogens, intercellular signaling controls virulence gene expression. However, in the intestinal pathogen Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium), although three signals, named AI-1, AI-2 and AI-3, have been described, their roles in virulence remain elusive. AI-3 is the 3,6- isomer of a previously described Vibrio cholerae signaling molecule; 3,5-dimethylpyrazin-2-ol (3,5-DPO). To elucidate the role of AI-3/DPO in S. Typhimurium, we have mapped the global transcriptomic responses to 3,5- and 3,6-DPO isomers in S. Typhimurium. Our studies showed that DPO affects expression of almost 8% of all genes. Specifically, expression of several genes involved in gut-colonization respond to DPO. Interestingly, most of the affected genes are similarly regulated by 3,5-DPO and 3,6-DPO, respectively, indicating that the two isomers have overlapping roles in S. Typhimurium.
由小分子介导的细菌细胞间信号传导，也称为自诱导物（AIs），能够使细菌根据环境条件做出同步行为反应。在许多细菌病原体中，细胞间信号传导控制着毒力基因的表达。然而，在肠道病原体肠炎沙门氏菌肠炎亚种鼠伤寒血清型（鼠伤寒沙门氏菌）中，尽管已经描述了三种信号，即AI-1、AI-2和AI-3，但它们在毒力中的作用仍不清楚。AI-3是先前描述的霍乱弧菌信号分子3,5-二甲基吡嗪-2-醇（3,5-DPO）的3,6-异构体。为了阐明AI-3/DPO在鼠伤寒沙门氏菌中的作用，我们绘制了鼠伤寒沙门氏菌对3,5-和3,6-DPO异构体的全局转录组反应图谱。我们的研究表明，DPO影响所有基因中近8%的基因表达。具体而言，一些参与肠道定植的基因的表达对DPO有反应。有趣的是，大多数受影响的基因分别受到3,5-DPO和3,6-DPO的类似调控，这表明这两种异构体在鼠伤寒沙门氏菌中具有重叠的作用。
