Institute of Biochemistry, University of Muenster, Corrensstraße 36, 48 149, Münster, Germany.
Angew Chem Int Ed Engl. 2023 Jun 5;62(23):e202301778. doi: 10.1002/anie.202301778. Epub 2023 Apr 17.
A major stage in the expression of genes is the translation of messenger RNA (mRNA), and the regulation of this process is essential for protein production in cells. How tightly controlled gene expression can be spatially and temporally, is particularly evident in polar cells and embryonic development. We need tools to dissect these complex processes, if we wish to understand the underlying links, especially the difficulties brought on by malfunction. External bioorthogonal triggers are very helpful in this area, if they let us precisely control where and when a process is started. Equipping nucleic acids with light-responsive groups has proven to be an effective approach to examine the dynamic regulatory route of mRNA translation in living cells. In this review, we present an overview of the most recent methods for optochemically controlling translation, focusing on cis-acting technologies.
基因表达的一个主要阶段是信使 RNA（mRNA）的翻译，而这个过程的调节对于细胞中蛋白质的产生是必不可少的。基因表达在空间和时间上的调控是如何紧密进行的，在极性细胞和胚胎发育中尤为明显。如果我们希望了解潜在的联系，特别是功能障碍带来的困难，就需要工具来剖析这些复杂的过程。如果外部生物正交触发物能够精确控制一个过程开始的位置和时间，那么它们在这方面非常有帮助。用光响应基团装备核酸已被证明是一种有效的方法，可用于在活细胞中检查 mRNA 翻译的动态调节途径。在这篇综述中，我们介绍了最近用于光化学控制翻译的方法，重点介绍顺式作用技术。
