Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Eco-Circular Agriculture; Guangdong Engineering Research Centre for Modern Eco-Agriculture; College of Natural Resources and Environment, South China Agricultural University, Guangzhou, China.
China Tobacco Guangxi Industrial Company Limited, Nanning, China.
Pol J Microbiol. 2023 Mar 24;72(1):47-60. doi: 10.33073/pjm-2023-009. Print 2023 Mar 1.
For decades chlorine dioxide has been used in water disinfection with excellent results. As the scope of application expands, chlorine dioxide has the potential for soil disinfection. We used amplicon sequencing and gas chromatography-mass spectrometry to compare the changes of four mixed rhizosphere microbial community samples and 12 tobacco leaf volatile samples four months after the flood irrigation with chlorine dioxide in different concentrations (0, 2, 4, 8 mg/l). Phenotypic data of 60 tobacco plants were also collected. The effects of chlorine dioxide on rhizosphere microorganisms were positively correlated with dose gradients. Bacteria responded more strongly in both community structure and metabolic pathways than fungi. Five new bacterial phyla (Firmicutes, Bacteroidota, Myxococcota, Patescibacteria, Verrucomicroboata) appeared in chlorine dioxide treatment groups, while the fungal community only appeared as one new fungal phylum (Basidomycota). Alterations in 271 predicted metabolic bacterial pathways were found. However, in the fungal community were only 10 alternations. The correlations between leaf volatile compounds and rhizosphere microorganisms under the influence of chlorine dioxide treatment could be observed based on network results. However, natural connectivity had already been declining rapidly when less than 20% of the network's nodes were removed. Therefore, the microbe-metabolite network is not stable. It might be why chlorine dioxide treatments did not significantly affect tobacco quality ( = 0.754) and phenotype ( = 0.867). As a comprehensive investigation of chlorine dioxide in agriculture, this study proves the effectiveness and safety of chlorine dioxide soil disinfection and widens the application range of chlorine dioxide.
几十年来，二氧化氯在水消毒方面的应用效果非常好。随着应用范围的扩大，二氧化氯有可能用于土壤消毒。我们使用扩增子测序和气相色谱-质谱联用技术，比较了在不同浓度（0、2、4、8 mg/L）二氧化氯洪水灌溉四个月后，四种混合根际微生物群落样本和 12 个烟草叶片挥发物样本的变化。还收集了 60 株烟草的表型数据。二氧化氯对根际微生物的影响与剂量梯度呈正相关。细菌在群落结构和代谢途径上的反应都比真菌强烈。在二氧化氯处理组中出现了五个新的细菌门（厚壁菌门、拟杆菌门、粘球菌门、Patescibacteria 门、疣微菌门），而真菌群落仅出现一个新的真菌门（担子菌门）。发现了 271 个预测代谢细菌途径的改变。然而，在真菌群落中只有 10 个变化。根据网络结果可以观察到在二氧化氯处理影响下叶片挥发物化合物与根际微生物之间的相关性。然而，当网络节点的不到 20%被移除时，自然连接性已经迅速下降。因此，微生物-代谢物网络并不稳定。这可能就是为什么二氧化氯处理对烟草质量（ = 0.754）和表型（ = 0.867）没有显著影响的原因。作为对农业中二氧化氯的综合调查，本研究证明了二氧化氯土壤消毒的有效性和安全性，并拓宽了二氧化氯的应用范围。
