Monteiro N O, Casanova M R, Fangueiro J F, Reis R L, Neves N M
3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal.
ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Biomed Mater. 2023 Apr 4;18(3). doi: 10.1088/1748-605X/acc55f.
The interaction between cells and biomaterials is essential for the success of biomedical applications in which the implantation of biomaterials in the human body is necessary. It has been demonstrated that material's chemical, mechanical, and structural properties can influence cell behaviour. The surface topography of biomaterials is a physical property that can have a major role in mediating cellmaterial interactions. This interaction can lead to different cell responses regarding cell motility, proliferation, migration, and even differentiation. The combination of biomaterials with mesenchymal stem cells (MSCs) for bone regeneration is a promising strategy to avoid the need for autologous transplant of bone. Surface topography was also associated with the capacity to control MSCs differentiation. Most of the topographies studied so far involve machine-generated surface topographies. Herein, our strategy differentiates from the above mentioned since we selected natural surface topographies that can modulate cell functions for regenerative medicine strategies.leaf was the selected topography to be replicated in polycaprolactone (PCL) membranes through polydimethylsiloxane moulding and using soft lithography. Afterwards, rat bone marrow stem cells (rBMSCs) were seeded at the surface of the imprinted PCL membranes to characterize the bioactive potential of our biomimetic surface topography to drive rBMSCs differentiation into the osteogenic lineage. The selected surface topography in combination with the osteogenic inductive medium reveals having a synergistic effect promoting osteogenic differentiation.
细胞与生物材料之间的相互作用对于生物医学应用的成功至关重要，在这些应用中，将生物材料植入人体是必要的。已经证明，材料的化学、机械和结构特性会影响细胞行为。生物材料的表面形貌是一种物理特性，在介导细胞与材料的相互作用中可能起主要作用。这种相互作用会导致细胞在运动性、增殖、迁移甚至分化方面产生不同的反应。将生物材料与间充质干细胞（MSCs）结合用于骨再生是一种有前景的策略，可避免自体骨移植的需求。表面形貌也与控制MSCs分化的能力有关。迄今为止研究的大多数形貌都涉及机器生成的表面形貌。在此，我们的策略与上述不同，因为我们选择了天然表面形貌，其可调节细胞功能以用于再生医学策略。树叶是通过聚二甲基硅氧烷模塑并使用软光刻技术在聚己内酯（PCL）膜中复制的选定形貌。之后，将大鼠骨髓干细胞（rBMSCs）接种在印迹PCL膜的表面，以表征我们的仿生表面形貌驱动rBMSCs向成骨谱系分化的生物活性潜力。选定的表面形貌与成骨诱导培养基相结合显示出具有促进成骨分化的协同作用。
