Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Nat Commun. 2023 Mar 17;14(1):1499. doi: 10.1038/s41467-023-37246-w.
Integrative analyses of transcriptomic and neuroimaging data have generated a wealth of information about biological pathways underlying regional variability in imaging-derived brain phenotypes in humans, but rarely in nonhuman primates due to the lack of a comprehensive anatomically-defined atlas of brain transcriptomics. Here we generate complementary bulk RNA-sequencing dataset of 819 samples from 110 brain regions and single-nucleus RNA-sequencing dataset, and neuroimaging data from 162 cynomolgus macaques, to examine the link between brain-wide gene expression and regional variation in morphometry. We not only observe global/regional expression profiles of macaque brain comparable to human but unravel a dorsolateral-ventromedial gradient of gene assemblies within the primate frontal lobe. Furthermore, we identify a set of 971 protein-coding and 34 non-coding genes consistently associated with cortical thickness, specially enriched for neurons and oligodendrocytes. These data provide a unique resource to investigate nonhuman primate models of human diseases and probe cross-species evolutionary mechanisms.
对转录组学和神经影像学数据的综合分析产生了大量关于人类成像衍生脑表型的区域变异性的生物学途径的信息，但由于缺乏全面的、解剖定义的灵长类动物脑转录组图谱，这种方法在非人类灵长类动物中很少使用。在这里，我们生成了来自 162 只食蟹猴的 819 个样本的互补批量 RNA 测序数据集和单细胞 RNA 测序数据集，以及神经影像学数据，以研究脑广泛基因表达与形态计量学的区域变化之间的联系。我们不仅观察到与人类相当的猕猴大脑的全脑/区域表达谱，而且还揭示了灵长类动物额叶内基因组合的背外侧-腹内侧梯度。此外，我们确定了一组 971 个编码蛋白和 34 个非编码基因与皮质厚度一致相关，特别是富含神经元和少突胶质细胞。这些数据为研究人类疾病的非人类灵长类动物模型和探索跨物种进化机制提供了独特的资源。
