Department of Obstetrics and Gynecology, Ohashi Hospital, Toho University, 2-22-36 Ohashi, Meguro-ku, Tokyo, 153-8515, Japan.
Department of Obstetrics and Gynecology, School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
Hum Cell. 2023 May;36(3):1199-1203. doi: 10.1007/s13577-023-00864-z. Epub 2023 Mar 17.
Human cervical carcinoma-derived cell lines have been frequently found to contain gangliosides with GM2-determinant, i.e., GM2, GalNAc-GM1b and GalNAc-GD1a, but GM2 was only detected in 5 of 15 tissues, and GalNAc-GM1b and GalNAc-GD1a were not found in any tissues from patients with several histological types of cervical carcinomas. To further characterize the ganglioside expression in cervical carcinomas, cells were grown by subcutaneous transplantation into nude mice, and gangliosides were quantitated by TLC-immunostaining with the anti-GM2 (YHD-06) antibody and a newly developed anti-GM3 (5H6) antibody, which reacts with GM3 and GM1b, but not with GD1a. Gangliosides with GM2-determinant in cells disappeared in transplanted cells, and the amount of GM3, a precursor for GM2, in transplanted cells was greater than in cultured cells. Also, transplanted cells containing GalNAc-GM1b newly expressed GM1b, suggesting that the activity of GalNAc transferase for synthesis of GalNAc-GM1b is retarded on subcutaneous transplantation. The ganglioside composition, with GM3 as the major one, in the transplanted cells was similar to that in cervical carcinoma tissues, and thus, the expression of gangliosides with GM2-determinant seemed to be accelerated under cell-cultivation conditions.
人宫颈癌衍生细胞系常被发现含有 GM2 决定簇的神经节苷脂，即 GM2、GalNAc-GM1b 和 GalNAc-GD1a，但 GM2 仅在 15 种组织中的 5 种中被检测到，而 GalNAc-GM1b 和 GalNAc-GD1a 在来自不同组织学类型宫颈癌患者的任何组织中均未被发现。为了进一步描述宫颈癌中神经节苷脂的表达，将细胞通过皮下移植到裸鼠中生长，并通过用抗 GM2（YHD-06）抗体和新开发的抗 GM3（5H6）抗体进行 TLC-免疫染色定量，该抗体与 GM3 和 GM1b 反应，但不与 GD1a 反应。具有 GM2 决定簇的神经节苷脂在移植细胞中的细胞中消失，GM2 的前体 GM3 在移植细胞中的量大于在培养细胞中的量。此外，含有 GalNAc-GM1b 的移植细胞新表达 GM1b，表明 GalNAc 转移酶合成 GalNAc-GM1b 的活性在皮下移植时受到抑制。移植细胞的神经节苷脂组成以 GM3 为主，与宫颈癌组织相似，因此，GM2 决定簇的神经节苷脂的表达似乎在细胞培养条件下得到加速。
