Jin Hong-Lei, Duan Sujuan, Zhang Pengxiang, Yang Ziyue, Zeng Yunping, Chen Ziqi, Hong Liu, Li Mengshu, Luo Lujun, Chang Zhenyi, Hu Jiliang, Wang Hong-Bin
Institute of Medical Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China; Guangzhou Key Laboratory of Chinese Medicine Research on Prevention and Treatment of Osteoporosis, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, No. 263, Longxi Avenue, Guangzhou, China.
Institute of Medical Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.
Cell Rep. 2023 Mar 28;42(3):112268. doi: 10.1016/j.celrep.2023.112268. Epub 2023 Mar 17.
The coordination of chloroplast and nuclear genome status is critical for plant cell function. Here, we report that Arabidopsis CHLOROPLAST AND NUCLEUS DUAL-LOCALIZED PROTEIN 1 (CND1) maintains genome stability in the chloroplast and the nucleus. CND1 localizes to both compartments, and complete loss of CND1 results in embryo lethality. Partial loss of CND1 disturbs nuclear cell-cycle progression and photosynthetic activity. CND1 binds to nuclear pre-replication complexes and DNA replication origins and regulates nuclear genome stability. In chloroplasts, CND1 interacts with and facilitates binding of the regulator of chloroplast genome stability WHY1 to chloroplast DNA. The defects in nuclear cell-cycle progression and photosynthesis of cnd1 mutants are respectively rescued by compartment-restricted CND1 localization. Light promotes the association of CND1 with HSP90 and its import into chloroplasts. This study provides a paradigm of the convergence of genome status across organelles to coordinately regulate cell cycle to control plant growth and development.
叶绿体与核基因组状态的协调对于植物细胞功能至关重要。在此，我们报道拟南芥叶绿体与细胞核双定位蛋白1（CND1）维持叶绿体和细胞核中的基因组稳定性。CND1定位于这两个区室，CND1的完全缺失导致胚胎致死。CND1的部分缺失扰乱核细胞周期进程和光合活性。CND1与核前复制复合体及DNA复制起点结合并调节核基因组稳定性。在叶绿体中，CND1与叶绿体基因组稳定性调节因子WHY1相互作用并促进其与叶绿体DNA的结合。通过区室限制的CND1定位分别挽救了cnd1突变体在核细胞周期进程和光合作用方面的缺陷。光促进CND1与热激蛋白90（HSP90）的结合及其导入叶绿体。本研究提供了一个细胞器间基因组状态汇聚以协调调节细胞周期来控制植物生长和发育的范例。
