NingboTech University, 315000, China.
NingboTech University, 315000, China.
Bioresour Technol. 2023 May;376:128907. doi: 10.1016/j.biortech.2023.128907. Epub 2023 Mar 16.
Heterotrophic nitrification and aerobic denitrification (HNAD) sludge were successfully acclimated. The effects of organics and dissolved oxygen (DO) on nitrogen and phosphorus removal by the HNAD sludge were investigated. The nitrogen can be heterotrophically nitrified and denitrified in the sludge at a DO of 6 mg/L. The TOC/N (total organic carbon to nitrogen) ratio of 3 was found to result in removal efficiencies of over 88% for nitrogen and 99% for phosphorus. The use of demand-driven aeration with a TOC/N ratio of 1.7 improved nitrogen and phosphorus removal from 35.68% and 48.17% to 68% and 93%, respectively. The kinetics analysis generated an empirical formula, Ammonia oxidation rate = 0.08917·(TOC·Ammonia)·Biomass. The nitrogen, carbon, glycogen, and poly-β-hydroxybutyric acid (PHB) metabolism pathways of HNAD sludge were constructed using the Kyoto Encyclopedia of Genes and Genomes (KEGG). The findings suggest that heterotrophic nitrification precedes aerobic denitrification, glycogen synthesis, and PHB synthesis.
成功驯化了异养硝化和好氧反硝化（HNAD）污泥。考察了有机物和溶解氧（DO）对 HNAD 污泥脱氮除磷的影响。在 DO 为 6mg/L 的条件下，污泥中的氮可以进行异养硝化和反硝化。发现 TOC/N（总有机碳与氮的比值）为 3 时，氮的去除率超过 88%，磷的去除率超过 99%。采用 TOC/N 比为 1.7 的需求驱动曝气，可以将氮和磷的去除率分别从 35.68%和 48.17%提高到 68%和 93%。动力学分析生成了一个经验公式，氨氧化速率=0.08917·（TOC·氨）·生物量。利用京都基因与基因组百科全书（KEGG）构建了 HNAD 污泥的氮、碳、糖原和聚-β-羟基丁酸（PHB）代谢途径。研究结果表明，异养硝化先于好氧反硝化、糖原合成和 PHB 合成。
