基于免疫调节和肠道菌群的不同相对分子质量羧甲基茯苓多糖的抗肿瘤活性
Antitumor Activity of Carboxymethyl Pachymaran with Different Molecular Weights Based on Immunomodulatory and Gut Microbiota.
机构信息
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Department of Nutrition, Food Science and Packaging, San Jose State University, San Jose, CA 95192, USA.
出版信息
Nutrients. 2023 Oct 25;15(21):4527. doi: 10.3390/nu15214527.
Carboxymethyl pachymaran (CMP) was treated via high-temperature and cellulase hydrolysis to obtain HTCMP, HTEC-24, and HTEC-48. The chemical structure and in vivo antitumor activities of the four types of CMPs were investigated. Compared with CMP (787.9 kDa), the molecular weights of HTCMP, HTEC-24, and HTEC-48 were decreased to 429.8, 129.9, and 68.6 kDa, respectively. The viscosities and particle sizes of the CMPs could also decrease with the decline in the molecular weights. All the CMPs showed antitumor abilities, but HTEC-24 exhibited the best activity. In the animal study, when curing the spleen and thymus, CMPs displayed immunomodulatory effects by increasing the secretion of IFN-γ and IL2 in mice. The CMPs also exerted an antitumor ability by regulating the gut microbiota in tumor-bearing mice. Our results established a foundation to develop an antitumor drug with CMP.
羧甲基几丁质（CMP）经过高温和纤维素酶水解处理，得到 HTCMP、HTEC-24 和 HTEC-48。研究了这四种 CMP 的化学结构和体内抗肿瘤活性。与 CMP（787.9 kDa）相比，HTCMP、HTEC-24 和 HTEC-48 的分子量分别降低至 429.8、129.9 和 68.6 kDa。CMP 的粘度和粒径也随分子量的降低而降低。所有 CMP 均表现出抗肿瘤能力，但 HTEC-24 的活性最好。在动物研究中，CMP 通过增加 IFN-γ 和 IL2 在小鼠中的分泌来发挥免疫调节作用，从而治疗脾和胸腺。CMP 还通过调节荷瘤小鼠的肠道微生物群发挥抗肿瘤作用。我们的研究结果为开发 CMP 抗肿瘤药物奠定了基础。
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