Department of Zoology, Maitreyi College, University of Delhi, Chanakyapuri, Delhi, 110021, India.
Department of Zoology, University of Delhi, Delhi, 110007, India.
Sci Rep. 2023 Mar 18;13(1):4470. doi: 10.1038/s41598-023-31271-x.
The FBN1 gene encodes profibrillin protein that is cleaved by the enzyme furin to release fibrillin-1 and a glucogenic hormone, asprosin. Asprosin is implicated in diverse metabolic functions as well as pathological conditions in mammals. However, till date, there are no studies on asprosin in any non-mammalian vertebrate. In this study, we have retrieved the spotted snakehead Channa punctata fbn1 gene (ss fbn1) from the testicular transcriptome data and validated it. The transcript is predicted to encode 2817 amino acid long putative profibrillin protein. Amino acid sequence alignment of deduced ss profibrillin with human profibrillin revealed that the furin cleavage site in profibrillin is well conserved in C. punctata. Further, differential expression of ss fbn1 was observed in various tissues with the highest expression in gonads. Prominent expression of furin was also observed in the gonads suggesting the possibility of proteolytic cleavage of profibrillin protein and secretion of asprosin in C. punctata. In addition, the C-terminal of the fbn1 gene of C. punctata that codes for asprosin protein has been cloned. Using in silico approach, physicochemical properties of the putative ss asprosin were characterized and post-translational changes were predicted. The putative ss asprosin protein sequence is predicted to consist of 142 amino acid residues, with conserved glycosylation sites. Further, the 3D model of ss asprosin was predicted followed by MD (molecular dynamics) simulation for energy minimization. Thus, the current study, for the first time in non-mammalian vertebrates, predicts and characterizes the novel protein asprosin using in silico approach.
该 FBN1 基因编码前纤维蛋白，该蛋白被酶原激活物释放酶切割，释放出原纤维蛋白-1 和一种糖生成激素，即 asprosin。Asprosin 参与哺乳动物的多种代谢功能以及病理状态。然而，迄今为止，在任何非哺乳动物脊椎动物中都没有关于 asprosin 的研究。在这项研究中，我们从睾丸转录组数据中检索到了斑点蛇 Channa punctata fbn1 基因（ss fbn1）并对其进行了验证。该转录本预测编码 2817 个氨基酸长的推定前纤维蛋白。推导的 ss 前纤维蛋白与人类前纤维蛋白的氨基酸序列比对表明，前纤维蛋白中的原激活酶切割位点在 C. punctata 中高度保守。此外，ss fbn1 在各种组织中的差异表达，在性腺中表达最高。在性腺中也观察到了 furin 的显著表达，这表明在 C. punctata 中可能存在前纤维蛋白蛋白的蛋白水解切割和 asprosin 的分泌。此外，还克隆了 C. punctata fbn1 基因的 C 端，该基因编码 asprosin 蛋白。通过计算方法，对推定的 ss asprosin 的理化性质进行了特征描述，并预测了翻译后变化。推定的 ss asprosin 蛋白序列预测由 142 个氨基酸残基组成，具有保守的糖基化位点。进一步，预测了 ss asprosin 的 3D 模型，然后进行 MD（分子动力学）模拟以进行能量最小化。因此，本研究首次在非哺乳动物脊椎动物中使用计算方法预测和表征了新型蛋白 asprosin。
