Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
Department of Animal Sciences, The Ohio State University, Wooster, OH 44691, USA.
Animal. 2023 Apr;17(4):100750. doi: 10.1016/j.animal.2023.100750. Epub 2023 Feb 28.
The regulation of adipose deposition in broiler chickens is an important factor for production efficiency to poultry producers and health concerns to customers. Although vitamin A and its metabolite [all-trans retinoic acid (atRA)] have been used for studies on adipogenesis in mammals and avian, effects of embryonic atRA on adipose development in embryonic (E) and posthatch (D) ages in broiler chickens have not been studied yet. Different concentrations of atRA (0 M-2 μM) were injected in broiler eggs at E10, and adipose tissues were sampled at E16. Percentages of adipose tissues in chicken embryos were significantly increased in the group injected with 500 nM of atRA compared to the 0 M group (P < 0.05). In addition, the adipocyte cross-sectional area (CSA) was significantly greater by in ovo injection of 500 nM atRA compared to the injection of 0 M (P < 0.01). Moreover, in ovo atRA-injected embryos were hatched and BWs were measured at D0, D7, and D14. BWs were not different from those of the 0 M group. Percentages of adipose tissues and CSA of the in ovo atRA-injected group (500 nM) were not different from those of the 0 M group at D14. Taken together, the current study clearly showed that in ovo injection of atRA promoted adipose deposition with hypertrophy during embryonic development, but its effects were not maintained in early posthatch age in broiler chickens, implying that embryonic atRA has an important role in the regulation of adipose development in chicken embryos.
调控肉鸡脂肪沉积对于禽类养殖者的生产效率和消费者的健康都非常重要。尽管维生素 A 及其代谢产物[全反式视黄酸(atRA)]已被用于哺乳动物和禽类的脂肪生成研究，但胚胎期(atRA)对肉鸡胚胎期(E)和出壳后(D)时期脂肪发育的影响尚未研究。在 E10 时，用不同浓度(atRA)的 2μM 注射到肉鸡卵中，并在 E16 时取样脂肪组织。与 0M 组相比，注射 500nM atRA 的鸡胚脂肪组织百分比显著增加(P<0.05)。此外，与注射 0M 的鸡胚相比，注射 500nM atRA 的鸡胚的脂肪细胞横截面积(CSA)显著增大(P<0.01)。此外，将鸡胚进行 atRA 注射，并在 D0、D7 和 D14 时测量出雏鸡体重(BW)。BW 与 0M 组无差异。注射 atRA 的鸡胚组(E16)的脂肪组织百分比和 CSA 与 0M 组在 D14 时无差异。总之，本研究清楚地表明，胚胎期注射 atRA 促进了胚胎发育过程中脂肪的沉积和肥大，但在肉鸡出壳后的早期阶段，其作用并未维持，这表明胚胎期 atRA 在调节鸡胚脂肪发育中具有重要作用。
