State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, 510060, China.
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, 510060, China; Guangdong Provincial Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
Biochem Biophys Res Commun. 2023 May 7;655:118-126. doi: 10.1016/j.bbrc.2023.03.029. Epub 2023 Mar 11.
Electroretinogram (ERG) is the most common clinical and basic visual electrodiagnostic test, which has long been used to evaluate the retinal function through photic stimulation. Despite its wide application, there are still some pitfalls often neglected in ERG recording, such as the recording time point, active electrode location, and the animal strain. In this study, we systematically analyzed and compared the effects of multiple factors on ERG, which would provide an important reference for ERG detection by other investigators. ERG was recorded using the Celeris D430 rodent ERG testing system. The amplitudes and latencies of a wave, b wave and oscillatory potentials (OPs) recorded from different electrode locations (subdermal and invasive), different times of day (day time 8:00 to 13:00 and night time 18:00 to 23:00), bilateral eyes (left and right), and different mouse strains (C57 and CD1) were analyzed and compared. Our results revealed that ERG was affected by active electrode locations and difference between day and night, while OPs seemed not to be influenced. There was no significant difference in the amplitudes or latencies of ERG and OPs between left and right eyes, irrespective of measurements at day or night, or which method was used. Compared to C57 mice, both ERG and OP responses were significantly decreased in Brn3b mice, a model for retinal ganglion cell (RGC) loss. In addition, there were some non-negligible differences in visual responses between C57 and CD1 mouse strains. Our results suggest that the invasive procedure is a reliable method for evaluating the visual function including VEP, ERG and OP responses in mice. Moreover, these comparative analyses provide valuable references for future studies of mammalian visual electrophysiology.
视网膜电图（ERG）是最常见的临床和基本视觉电诊断测试，长期以来一直用于通过光刺激评估视网膜功能。尽管它的应用广泛，但在 ERG 记录中仍然存在一些经常被忽视的陷阱，例如记录时间点、活动电极位置和动物品系。在这项研究中，我们系统地分析和比较了多种因素对 ERG 的影响，这将为其他研究人员进行 ERG 检测提供重要参考。ERG 使用 Celeris D430 啮齿动物 ERG 测试系统进行记录。分析和比较了不同电极位置（皮下和侵入性）、不同时间（白天 8:00 至 13:00 和夜间 18:00 至 23:00）、双眼（左眼和右眼）和不同品系（C57 和 CD1）记录的 a 波、b 波和振荡电位（OPs）的幅度和潜伏期。我们的结果表明，ERG 受到活动电极位置和昼夜差异的影响，而 OPs 似乎不受影响。无论在白天还是晚上，无论使用哪种方法，左眼和右眼的 ERG 和 OPs 的幅度或潜伏期均无显著差异。与 C57 小鼠相比，Brn3b 小鼠（视网膜神经节细胞（RGC）丢失模型）的 ERG 和 OP 反应均显著降低。此外，C57 和 CD1 小鼠品系之间的视觉反应存在一些不可忽视的差异。我们的结果表明，侵入性程序是一种可靠的方法，可用于评估包括 VEP、ERG 和 OP 反应在内的小鼠视觉功能。此外，这些比较分析为未来的哺乳动物视觉电生理学研究提供了有价值的参考。
