Meng Xingyu, Guo Ping, Li Jian, Huang Haikang, Li Zeqi, Yan Hailong, Chu Zonglin, Zhou Yi-Ge
Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Biosens Bioelectron. 2023 May 15;228:115203. doi: 10.1016/j.bios.2023.115203. Epub 2023 Mar 7.
In this work, we report a versatile and tunable platform for the construction of various cell array biochips using a simple soft lithographic approach to pattern polydopamine (PDA) arrays via microcontact printing (μCP). Instead of direct polymerization of PDA on the polydimethylsiloxane (PDMS) tips, dopamine monomers were first printed on the substrate followed by a self-oxidative polymerization step facilitated by ammonia vapor to grow PDA in situ, which greatly reduced the reaction time and prevented the PDMS tips from damaging. The improved robustness and utility of the PDMS tips allows the formation of tunable PDA array chips with controllable PDA feature size and shape. As a result, single cell, multi-cells and cell line arrays can be constructed. The obtained cell array chips showed high single cell capture efficiency, providing a standardized single cell array analysis platform. Meanwhile, the adhered cells can maintain excellent viability and proliferation ability on the PDA chips. Moreover, a cytotoxicity sensor with single cell resolution was enabled on the single cell array chip. This work provides a promising cell array biochip platform for high-throughput cellular analysis and cell screening.
在本工作中，我们报道了一种通用且可调谐的平台，用于构建各种细胞阵列生物芯片。该平台采用简单的软光刻方法，通过微接触印刷（μCP）对聚多巴胺（PDA）阵列进行图案化。与在聚二甲基硅氧烷（PDMS）尖端直接聚合PDA不同，多巴胺单体首先被印刷在基底上，随后在氨蒸汽促进下进行自氧化聚合步骤，以原位生长PDA，这大大缩短了反应时间并防止了PDMS尖端受损。PDMS尖端改进后的耐用性和实用性使得能够形成具有可控PDA特征尺寸和形状的可调谐PDA阵列芯片。结果，可以构建单细胞、多细胞和细胞系阵列。所获得的细胞阵列芯片显示出高单细胞捕获效率，提供了一个标准化的单细胞阵列分析平台。同时，粘附在PDA芯片上的细胞能够保持优异的活力和增殖能力。此外，在单细胞阵列芯片上实现了具有单细胞分辨率的细胞毒性传感器。这项工作为高通量细胞分析和细胞筛选提供了一个有前景的细胞阵列生物芯片平台。
