Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Small. 2018 Sep;14(38):e1802128. doi: 10.1002/smll.201802128. Epub 2018 Aug 21.
Here, a negative microcontact printing method is developed to form hydrophilic polydopamine (PDA) patterns with micrometer resolution on hydrophobic including perfluorinated surfaces. In the process of the negative microcontact printing, a uniform PDA thin film is first formed on the hydrophobic surface. An activated polydimethylsiloxane (PDMS) stamp is then placed in contact with the PDA-coated hydrophobic surface. Taking advantage of the difference in the surface energy between the hydrophobic surface and the stamp, PDA is removed from the contact area after the stamp release. As a result, a PDA pattern complementary to the stamp is obtained on the hydrophobic surface. By using the negative microcontact printing, arrays of liquid droplets and single cells are reliably formed on perfluorinated surfaces. Microlens array with tunable focal length for imaging studies is further created based on the droplet array. The negative microcontact printing method is expected to be widely applicable in high-throughput chemical and biological screening and analysis.
在这里，开发了一种负面微接触印刷方法，可在包括全氟表面在内的疏水性表面上形成具有亚微米分辨率的亲水性聚多巴胺（PDA）图案。在负面微接触印刷过程中，首先在疏水性表面上形成均匀的 PDA 薄膜。然后将经过活化的聚二甲基硅氧烷（PDMS）印章与 PDA 涂层的疏水性表面接触。利用疏水性表面和印章之间的表面能差异，在印章释放后，从接触区域去除 PDA。结果，在疏水性表面上获得与印章互补的 PDA 图案。通过使用负面微接触印刷，可以在全氟表面上可靠地形成液滴阵列和单细胞。进一步基于液滴阵列创建了具有可调焦距的微透镜阵列，用于成像研究。预计负面微接触印刷方法将广泛应用于高通量化学和生物筛选和分析。
