Shinbo T, Yamaguchi T, Nishimura K, Sugiura M
National Chemical Laboratory for Industry, Ibaraki, Japan.
J Chromatogr. 1987 Sep 18;405:145-53. doi: 10.1016/s0021-9673(01)81756-8.
Chromatographic packings for optical resolution of underivatized racemic amino acids were prepared by dynamic coating of a chiral crown ether on reversed-phase packings, and their characteristics were examined. Almost all the amino acids commonly found in proteins were finely separated into their enantiomers when a dilute aqueous solution of perchloric acid was used as the eluent. The enantiomers forming more stable complexes with the chiral crown ether were eluted after those forming less stable complexes. The retention and resolution of racemic amino acids depended on the eluent, the column temperature and the amount of crown ether coated. The separation coefficients, alpha, increased with decreasing column temperature and/or with increasing amount of crown ether coated.
通过在手性冠醚在反相填料上进行动态涂覆，制备了用于未衍生化外消旋氨基酸光学拆分的色谱填料，并对其特性进行了研究。当使用高氯酸稀水溶液作为洗脱剂时，蛋白质中常见的几乎所有氨基酸都能被精细地拆分为对映体。与手性冠醚形成较稳定配合物的对映体在形成较不稳定配合物的对映体之后被洗脱。外消旋氨基酸的保留和拆分取决于洗脱剂、柱温以及冠醚的涂覆量。分离系数α随着柱温降低和/或冠醚涂覆量增加而增大。
