Kaniwa M A
National Institute of Hygienic Sciences, Tokyo, Japan.
J Chromatogr. 1987 Sep 18;405:263-71. doi: 10.1016/s0021-9673(01)81767-2.
A method was developed for the high-performance liquid chromatographic determination of dithiocarbamates (DTCs), after conversion of zinc dithiocarbamates into the corresponding cobalt(III) complexes. When shaking a chloroform-acetone extract from a rubber sample with cobalt(II) chloride, mixed-ligand cobalt(III) complexes were often formed because of the coexistence of more than two kinds of zinc dithiocarbamates in the rubber sample. The chromatograms were so complicated that DTCs could not easily be determined in the usual manner. However, it was possible to identify and to quantify DTCs by comparing retention times and relative intensities of the multiple peaks of the cobalt(III) complexes obtained from standard mixtures of zinc dithiocarbamates.
开发了一种高效液相色谱法，用于测定二硫代氨基甲酸盐（DTCs），该方法是先将二硫代氨基甲酸锌转化为相应的钴（III）配合物。当用氯化钴（II）振荡橡胶样品的氯仿 - 丙酮提取物时，由于橡胶样品中存在两种以上的二硫代氨基甲酸锌，常常会形成混合配体钴（III）配合物。色谱图非常复杂，以至于无法以常规方式轻松测定DTCs。然而，通过比较从二硫代氨基甲酸锌标准混合物中获得的钴（III）配合物的多个峰的保留时间和相对强度，可以鉴定和定量DTCs。
