尿液和血浆中糖胺聚糖己糖胺的气相色谱定量测定。

Quantitative gas chromatographic measurement of glycosaminoglycan hexosamines in urine and plasma.

作者信息

Larking P W

机构信息

Department of Pathology Services, Dunedin Public Hospital, New Zealand.

出版信息

J Chromatogr. 1987 Sep 25;420(2):231-9. doi: 10.1016/0378-4347(87)80180-9.

DOI:10.1016/0378-4347(87)80180-9

PMID:3693498

Abstract

A method is described for the quantitative determination of urine and plasma glycosaminoglycans (GAGs) by gas chromatography of the acetylated amino sugars. GAGs were first recovered by precipitation from urine with alkyltrimethylammonium bromide and from plasma by mini-column chromatography after papain digestion. Urine samples (24) analysed for total hexosamines by gas chromatography and for uronic acid by colorimetry had a correlation coefficient of 0.85. The within-run coefficient of variation (C.V.) for nineteen samples from a pooled urine was 5.2% for total hexosamines and that for the ratio of galactosamine to total hexosamines was 3.7%. The corresponding C.V. values for twelve plasma samples from a common pool were 6.5 and 3.7%. The mean ratio of galactosamine to total hexosamine in ten pre-breakfast spot urines was 51.5%. The corresponding ratio in the plasma from twenty adolescent blood donors was 76.3% and the mean total hexosamine content of the GAGs was 47.36 mumol/l.

摘要

本文描述了一种通过乙酰化氨基糖的气相色谱法对尿液和血浆中的糖胺聚糖（GAGs）进行定量测定的方法。首先，通过用烷基三甲基溴化铵从尿液中沉淀以及在木瓜蛋白酶消化后通过微型柱色谱法从血浆中回收GAGs。通过气相色谱法分析24份尿液样本中的总己糖胺，并通过比色法分析糖醛酸，其相关系数为0.85。来自混合尿液的19个样本中，总己糖胺的批内变异系数（C.V.）为5.2%，半乳糖胺与总己糖胺的比率的变异系数为3.7%。来自同一个混合样本的12份血浆样本的相应C.V.值分别为6.5%和3.7%。10份早餐前即时尿样中半乳糖胺与总己糖胺的平均比率为51.5%。20名青少年献血者血浆中的相应比率为76.3%，GAGs的平均总己糖胺含量为47.36μmol/l。