在中性单糖和糖醛酸存在下对己糖胺进行气相色谱分析的简便方法。
Convenient method for the gas chromatographic analysis of hexosamines in the presence of neutral monosaccharides and uronic acids.
作者信息
Honda S, Kakehi K, Okada K
出版信息
J Chromatogr. 1979 Sep 1;176(3):367-73. doi: 10.1016/s0021-9673(00)89454-6.
PMID:546920
Abstract
A convenient gas chromatographic method has been devised for the analysis of hexosamines in the presence of neutral and acidic sugars, which involves sequential derivatization reactions of nitrous acid deamination, mercaptalation, and trimethylsilylation. This method allows rapid, simultaneous determination of 0.1-1 micromole samples of hexosamines with coefficients of variation of less than 3%.
摘要
已设计出一种便捷的气相色谱法，用于在中性糖和酸性糖存在的情况下分析己糖胺，该方法涉及亚硝酸脱氨、缩硫醛化和三甲基硅烷化的顺序衍生反应。此方法能够快速同时测定0.1 - 1微摩尔的己糖胺样品，变异系数小于3%。
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