State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, People's Republic of China; State Key Laboratory of Photon-Technology in Western China Energy, International Collaborative Center on Photoelectric Technology and Nano Functional Materials, School of Physics, Institute of Photonics&Photon Technology, Northwest University, Xi'an, 710069, People's Republic of China.
State Key Laboratory of Photon-Technology in Western China Energy, International Collaborative Center on Photoelectric Technology and Nano Functional Materials, School of Physics, Institute of Photonics&Photon Technology, Northwest University, Xi'an, 710069, People's Republic of China.
Anal Chim Acta. 2023 Apr 29;1252:341051. doi: 10.1016/j.aca.2023.341051. Epub 2023 Mar 6.
A reflective fiber-optic Fabry-Perot cavity probe sensor is proposed to selectively measure cholesterol concentration by insert single mode fiber into ceramic tube and immobilize epoxy resin (ER)/graphene oxide (GO)/beta-cyclodextrin (β-CD) multi-layer film onto end face of ceramic tube. EDC/NHS activated GO is selected to form chemical binding with β-CD, and β-CD is the sensitive materials to bind with cholesterol molecules. With multi-layer film assisted, the sensitivity of sensor to cholesterol concentration can reach 3.92 nm/mM and the limit of detection reaches 3.48 μ M. In addition, 4 mM hemoglobin, glucose and ascorbic acid are doped into a set cholesterol sample and verified the highly selectivity of sensing cholesterol. Furthermore, the reproducibility was proved by measure the spectrum of four sensors with same fabrication process, and the reusability was also proved by repeated measurements. Overall, the sensor features with high mechanical strength, ease of fabrication, real-time monitoring, low cost and ease for measurement that given by probe structure. Therefore, the sensor provides a remarkable analytical platform for biosensing applications.
提出了一种反射光纤法布里-珀罗腔探头传感器，通过将单模光纤插入陶瓷管并将环氧树脂（ER）/氧化石墨烯（GO）/β-环糊精（β-CD）多层膜固定在陶瓷管的端面上，选择性地测量胆固醇浓度。选择 EDC/NHS 激活的 GO 与 β-CD 形成化学结合，β-CD 是与胆固醇分子结合的敏感材料。在多层膜的辅助下，传感器对胆固醇浓度的灵敏度可达 3.92nm/mM，检测限达到 3.48μM。此外，在一组胆固醇样品中掺杂了 4mM 的血红蛋白、葡萄糖和抗坏血酸，验证了传感器对胆固醇的高选择性。此外，通过测量四个具有相同制造工艺的传感器的光谱，证明了传感器的重现性，通过重复测量，证明了传感器的可重复性。总的来说，该传感器具有探头结构赋予的高机械强度、易于制造、实时监测、低成本和易于测量等特点。因此，该传感器为生物传感应用提供了一个显著的分析平台。
