Kaha Marshila, Noda Masayoshi, Maeda Yoshiaki, Kaneko Yumika, Yoshino Tomoko, Tanaka Tsuyoshi
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
J Biosci Bioeng. 2023 May;135(5):359-368. doi: 10.1016/j.jbiosc.2023.01.006. Epub 2023 Mar 17.
Oil body-associated proteins from the oleaginous diatom Fistulifera solaris were identified by proteomic analysis of oil bodies of various sizes (small, middle, and large) by time-dependent culturing upon nutrient-starvation at 36, 96 and 168 h. This diatom strain has the capability to accumulate neutral lipids and triacylglycerol. Liquid chromatography-tandem mass spectrometry analysis revealed 662 proteins in all oil body sizes. Among these, 132 proteins were predicted to be localized to the endoplasmic reticulum. Seventeen proteins that exhibited a positive correlation with gene expression and the oil body size were selected as novel candidates for oil body-associated proteins. Among the 17 protein candidates, two proteins encoded by fso:g8246 and fso:g10200 were confirmed to be localized on the surface of the oil body and endoplasmic reticulum. A protein encoded by fso:g2514, which is involved in sterol biosynthesis, was also identified. This protein was likely to localize to mitochondria; however, inhibitor assays suggested that it might play a role in lipid degradation. Our work provides new insights into the proteomics of microalgae and provides a valuable strategy for boosting lipid productivity in microalgae.
通过对在36、96和168小时营养饥饿条件下随时间培养的不同大小（小、中、大）油体进行蛋白质组学分析，鉴定了来自产油硅藻太阳瘘管藻的油体相关蛋白。该硅藻菌株具有积累中性脂质和三酰甘油的能力。液相色谱-串联质谱分析在所有油体大小中鉴定出662种蛋白质。其中，132种蛋白质被预测定位于内质网。选择了17种与基因表达和油体大小呈正相关的蛋白质作为油体相关蛋白的新候选物。在这17种候选蛋白质中，由fso:g8246和fso:g10200编码的两种蛋白质被证实定位于油体和内质网表面。还鉴定了一种由fso:g2514编码的参与甾醇生物合成的蛋白质。该蛋白质可能定位于线粒体；然而，抑制剂试验表明它可能在脂质降解中起作用。我们的工作为微藻蛋白质组学提供了新的见解，并为提高微藻脂质生产力提供了有价值的策略。
