Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Biotechnology Laboratory, Electric Power Development CO. Ltd, Yanagisaki-machi, Wakamatsu-ku, Kitakyushu 808-0111, Japan.
Bioresour Technol. 2017 Dec;245(Pt B):1520-1526. doi: 10.1016/j.biortech.2017.05.188. Epub 2017 Jun 1.
This study investigated the induction of oil synthesis in the oleaginous diatom, Fistulifera solaris, following irradiation with small doses of UV-C. A rapid induction of oil accumulation was confirmed within 6h following UV-C radiation of the diatom cells, with increases in cell oil body volumes after 24h of approximately 4- to 6-fold from the initial volume. Reactive oxygen species (ROS), which can be generated by a UV-C-mediated reaction, were detected in irradiated cells and the correlation between ROS generation and oil accumulation was confirmed. The smallest UV-C intensity required for oil induction in the cells was 10mJ/cm. Based on the ideal biodiesel profile, the most suitable FAME composition was obtained when UV was used to irradiate the cells. The UV-C radiation method is therefore a solution for shortening the oil accumulation period and improving biodiesel productivity.
本研究探讨了小剂量 UV-C 辐射对产油硅藻 Fistulifera solaris 中油脂合成的诱导作用。结果表明，UV-C 辐射后 6 小时内即可迅速诱导油脂积累，24 小时后细胞油体体积增加约 4-6 倍。UV-C 介导的反应会产生活性氧（ROS），在照射细胞中检测到了 ROS，证实了 ROS 的产生与油脂积累之间存在相关性。细胞中诱导油脂所需的最小 UV-C 强度为 10mJ/cm。基于理想的生物柴油特性，发现使用 UV 照射细胞时可以获得最适宜的 FAME 组成。因此，UV-C 辐射方法是缩短油脂积累周期和提高生物柴油产量的一种解决方案。
