用于从人和动物粪便中初次分离气单胞菌属的选择性培养基的比较。
Comparison of selective media for primary isolation of Aeromonas species from human and animal feces.
作者信息
Mishra S, Nair G B, Bhadra R K, Sikder S N, Pal S C
机构信息
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
出版信息
J Clin Microbiol. 1987 Nov;25(11):2040-3. doi: 10.1128/jcm.25.11.2040-2043.1987.
Five selective media were evaluated for their effectiveness in the primary isolation of Aeromonas spp. from human patients with acute diarrhea and from healthy domestic animals. Sheep blood agar with 30 mg of ampicillin per liter (ASBA30) yielded a significantly higher percentage of positive specimens as compared with the four other media. The effective combination of two selective media with which 98% of all isolates were detected and with which all of the three human-associated Aeromonas spp. could be isolated was ASBA30-DNase-toluidine blue agar. ASBA30 was the most sensitive medium since it permitted more growth of Aeromonas colonies and effectively suppressed competing microflora. We recommend the use of ASBA30-DNase-toluidine blue agar for investigations in which an attempt is made to assess the significance of Aeromonas spp. in the etiology of human diarrhea.
对五种选择性培养基进行了评估，以确定它们从急性腹泻患者和健康家畜中初步分离气单胞菌属细菌的有效性。每升含30毫克氨苄青霉素的羊血琼脂（ASBA30）与其他四种培养基相比，阳性标本的百分比显著更高。两种选择性培养基的有效组合（用其检测出了所有分离菌株的98%，且能分离出所有三种与人类相关的气单胞菌属细菌）是ASBA30-脱氧核糖核酸酶-甲苯胺蓝琼脂。ASBA30是最敏感的培养基，因为它能使气单胞菌菌落生长得更多，并有效抑制竞争性微生物群落。我们建议使用ASBA30-脱氧核糖核酸酶-甲苯胺蓝琼脂进行调查，以评估气单胞菌属细菌在人类腹泻病因中的重要性。
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