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为梨火疫病菌构建高质量基因组组装和注释资源,该病菌会导致果实溃疡。

High-Quality Genome Assembly and Annotation Resource of , Causing Fruit Scab on .

机构信息

State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

出版信息

Plant Dis. 2023 Oct;107(10):3264-3268. doi: 10.1094/PDIS-10-22-2322-A. Epub 2023 Oct 13.

Abstract

is a fungal pathogen that causes fruit scab disease in the edible-oil (tea oil) plant ( Abel). The absence of genome resources for this fungus hampers functional genetic studies of the pathogenesis mechanism of . This study reports the genome assembly of strain SM-YC-2 collected from tea oil tree fruit with scab disease in Fujian Province, China. Combining 16.44 Gb of PacBio Sequel II long reads and 5.13 Gb of Illumina NovaSeq reads, we generated a 25.93-Mb (99.19% of expected genome size) high-quality genome assembly with 52.66% GC content, 5.05% repeats, and over 98% Benchmarking Universal Single-Copy Orthologs completeness for strain SM-YC-2. These high-quality genome assembly resources will facilitate functional genomic characterization studies, enhance insights into the pathogenicity mechanism of , and support the development of molecular-based control strategies.

摘要

是一种真菌病原体,可导致食用油(茶油)植物(油茶)的果实疮痂病。由于缺乏该真菌的基因组资源,限制了对其致病机制的功能遗传研究。本研究报道了从中国福建省患疮痂病的油茶果实中采集的菌株 SM-YC-2 的基因组组装。结合 16.44 Gb 的 PacBio Sequel II 长读长和 5.13 Gb 的 Illumina NovaSeq 读长,我们生成了一个 25.93-Mb(预期基因组大小的 99.19%)的高质量基因组组装,GC 含量为 52.66%,重复序列为 5.05%,超过 98%的基准通用单拷贝直系同源物完整性用于菌株 SM-YC-2。这些高质量的基因组组装资源将有助于功能基因组特征研究,增强对致病机制的认识,并支持基于分子的控制策略的发展。

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