Butler W R, Kilburn J O, Kubica G P
Mycobacteriology Laboratory, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1987 Nov;25(11):2126-31. doi: 10.1128/jcm.25.11.2126-2131.1987.
High-performance liquid chromatography analysis of the p-bromophenacyl esters of mycolic acids from whole organisms gave chromatographic patterns that were useful in differentiation of Rhodococcus and Nocardia species. Rhodococcus equi, R. erythropolis, and R. rhodochrous contained more-polar mycolic acids and were easily separated from the less-polar mycolic acid-containing species of R. sputi, R. bronchialis, R. corallinus, R. rubropertinctus, and R. terrae. The less-polar mycolic acid-containing Rhodococcus species showed chromatographic patterns that partially overlapped (in elution times) the patterns of Nocardia asteroides, N. otitidiscaviarum, and N. brasiliensis, but the larger number of peaks in the last species made separation between the genera possible. Distinct chromatographic patterns were found for most species, except for R. equi strains that showed two different patterns. Strains of R. rubropertinctus and R. terrae appeared identical. N. asteroides and N. otitidiscaviarum showed similar mycolic acid patterns.
对来自完整生物体的分枝菌酸对溴苯甲酰酯进行高效液相色谱分析，得到的色谱图有助于区分红球菌属和诺卡氏菌属的菌种。马红球菌、红平红球菌和红串红球菌含有极性更强的分枝菌酸，很容易与含极性较弱分枝菌酸的痰红球菌、支气管红球菌、珊瑚红球菌、红赭红球菌和土壤红球菌区分开来。含极性较弱分枝菌酸的红球菌属菌种的色谱图在洗脱时间上与星形诺卡氏菌、耳炎诺卡氏菌和巴西诺卡氏菌的色谱图部分重叠，但最后一个菌种的峰数较多，使得这两个属之间的分离成为可能。除马红球菌菌株呈现两种不同的色谱图外，大多数菌种都有明显的色谱图。红赭红球菌和土壤红球菌的菌株看起来相同。星形诺卡氏菌和耳炎诺卡氏菌的分枝菌酸模式相似。
