Mir Ashaq Hussain, Iqbal Mir Khurshid, Banday Mujeeb Zafar, Balkhi Henah Mehraj, Haq Ehtishamul
Department of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Department of Biochemistry, Government Medical College, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Curr Ther Res Clin Exp. 2023 Feb 14;98:100695. doi: 10.1016/j.curtheres.2023.100695. eCollection 2023.
Glial tumors are the most common primary malignant central nervous system tumors. They are hard to treat, not only because of the deregulation in multiple pathways but also because they are not contained in a well-defined mass with clear borders. The use of a single therapeutic agent to target gliomas has yielded unsatisfactory results.
A combination of molecules targeting multiple pathways may prove to be a better alternative.
The effect of caffeic acid phenethyl ester and crocin on the proliferation and death of U87-MG cells over a concentration range was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. A colony formation assay was used to measure the effect of caffeic acid phenethyl ester and crocin on contact inhibition and anchorage independence ability of U87-MG cells. Furthermore, apoptosis in U87-MG cells was analyzed by propidium iodide assay. Real-time polymerase chain reaction and Western blotting were performed to determine the expression level of p53, epidermal growth factor receptor, and proliferating cell nuclear antigen.
Caffeic acid phenethyl ester and crocin when used in combination present an anticancer potential for glioma. These molecules, in combination, inhibit proliferation and induce apoptosis in U87-MG glioma cells. Our results provide evidence that combination treatment realigns the expression paradigm of p53, epidermal growth factor receptor, and proliferating cell nuclear antigen in cotreated U87-MG cells.
The combination of caffeic acid phenethyl ester and crocin led to inhibition in glioma cell proliferation and might prove to be an effective adjunct to the therapies in vogue.
胶质细胞瘤是最常见的原发性中枢神经系统恶性肿瘤。它们难以治疗，不仅因为多种信号通路失调，还因为它们并非局限于边界清晰的明确肿块中。使用单一治疗药物靶向胶质瘤的效果并不理想。
联合使用靶向多种信号通路的分子可能是更好的选择。
采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐和乳酸脱氢酶检测法，分析咖啡酸苯乙酯和藏红花素在一定浓度范围内对U87-MG细胞增殖和死亡的影响。采用集落形成试验检测咖啡酸苯乙酯和藏红花素对U87-MG细胞接触抑制和锚定非依赖性能力的影响。此外，通过碘化丙啶检测法分析U87-MG细胞的凋亡情况。进行实时聚合酶链反应和蛋白质免疫印迹法以确定p53、表皮生长因子受体和增殖细胞核抗原的表达水平。
咖啡酸苯乙酯和藏红花素联合使用对胶质瘤具有抗癌潜力。这些分子联合使用可抑制U87-MG胶质瘤细胞的增殖并诱导其凋亡。我们的结果表明，联合治疗可使联合处理的U87-MG细胞中p53、表皮生长因子受体和增殖细胞核抗原的表达模式重新调整。
咖啡酸苯乙酯和藏红花素联合使用可抑制胶质瘤细胞增殖，可能是当前流行疗法的有效辅助手段。
