Mohamad Noha, Khedr Ahmed M B, Shaker Olfat Gamil, Hassan Mohammed
Department of Dermatology, Faculty of Medicine, Fayoum University, Faiyum, Egypt.
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt.
Skin Appendage Disord. 2023 Mar;9(2):111-120. doi: 10.1159/000527851. Epub 2023 Feb 2.
Alopecia areata (AA) is a common autoimmune condition that affects anagen hair follicles. The most commonly recognized theory is that it is a T-cell-mediated autoimmune disorder in a genetically susceptible individual. MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) were thought to play a function in the pathogenesis. The expression of lncRNA HOTAIR and miRNA-205 and their relation to transforming growth factor β1 (TGF-β1) in AA were not studied.
The aim of the studywas to evaluate the role of miRNA-205, lncRNA, HOTAIR, and TGF-β1 levels in AA pathogenesis, clinical course, and severity of AA.
Two groups of subjects were included in this case-control study: 50 patients with AA and 50 healthy matched controls. miRNA-205 and lncRNA HOTAIR expression levels were assayed using quantitative RT-PCR, while serum levels of TGF-β1 were assayed using ELISA techniques.
The serum expression of lncRNA HOTAIR was significantly downregulated in AA patients with a value < 0.001, while the serum expression of both miRNA-205 and TGF-β1 were significantly upregulated in patients.
DISCUSSION/CONCLUSION: This study highlights the potential role of high serum expression of miRNA-205 and TGF-β1 and the low serum expression of lncRNA HOTAIR in AA pathogenesis. This could be used as a therapeutic target to treat AA.
斑秃（AA）是一种常见的自身免疫性疾病，会影响生长期毛囊。最被广泛认可的理论是，在具有遗传易感性的个体中，它是一种T细胞介导的自身免疫性疾病。微小RNA（miRNA）和长链非编码RNA（lncRNA）被认为在发病机制中发挥作用。尚未研究lncRNA HOTAIR和miRNA - 205的表达及其与斑秃中转化生长因子β1（TGF -β1）的关系。
本研究的目的是评估miRNA - 205、lncRNA、HOTAIR和TGF -β1水平在斑秃发病机制、临床病程及严重程度中的作用。
本病例对照研究纳入两组受试者：50例斑秃患者和50例健康匹配对照。使用定量逆转录聚合酶链反应（RT - PCR）检测miRNA - 205和lncRNA HOTAIR的表达水平，同时使用酶联免疫吸附测定（ELISA）技术检测血清中TGF -β1的水平。
lncRNA HOTAIR的血清表达在斑秃患者中显著下调，P值<0.001，而miRNA - 205和TGF -β1的血清表达在患者中均显著上调。
讨论/结论：本研究强调了血清中miRNA - 205和TGF -β1高表达以及lncRNA HOTAIR低表达在斑秃发病机制中的潜在作用。这可作为治疗斑秃的一个治疗靶点。
