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氧化脂质，一种来自伊朗里海眼镜蛇Oxiana的新型抗癌磷脂酶A2样蛋白。

Oxilipin, a New Anti-cancer Phospholipase A2-like Protein from Iranian Caspian Cobra, Oxiana.

作者信息

Shekarabi Seyed Mahdi, Parsian Hadi, Pooshang Bagheri Kamran, Shahbazzadeh Delavar

机构信息

Biotechnology Research Center, Medical Biotechnology Department, Venom and Biotherapeutics Molecules Lab., Pasteur Institute of Iran, Tehran, Iran.

Department of Clinical Biochemistry, Babol University of Medical Sciences, Babol, Iran.

出版信息

Iran J Pharm Res. 2022 Dec 20;21(1):e129616. doi: 10.5812/ijpr-129616. eCollection 2022 Dec.

DOI:10.5812/ijpr-129616
PMID:36937210
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10016118/
Abstract

The discovery of novel anti-cancer agents from natural resources is highly necessary. Concerning the above problem, the purpose of this study was to discover an anti-cancer compound from Caspian cobra venom. Fractionation of Caspian cobra venom was performed by gel filtration and IEX chromatography. The results showed an anti-cancer protein nominated as Oxilipin. Activity and toxicity of Oxilipin were studied on the colon SW480 cancer cell line using MTT, LDH release, PI staining, morphological cell analysis, hemolysis, and anti-proliferation assays. Oxilipin, an 11kDa protein purified from the venom of the Caspian cobra. LC/MS/MS analysis of obtained protein showed homology with Phospholipase A2 from ‎. 40 µg/ml of Oxilipin can induce an apoptotic effect in SW480 cell line up to 90%; meanwhile, this amount can induce only one-third of cytotoxicity on a normal cell. In this study, Iranian cobra venom was found to have cytotoxic effects on SW480 colon cancer tumor cells, with the least cytotoxicity on normal cells on HEK-293. Given that Oxilipin has slight toxicity on normal cells, it can be hypothesized that the obtained peptide can be considered as a drug lead in an animal model study of colon cancer.

摘要

从自然资源中发现新型抗癌药物非常必要。针对上述问题，本研究的目的是从里海眼镜蛇毒液中发现一种抗癌化合物。通过凝胶过滤和离子交换色谱法对里海眼镜蛇毒液进行分离。结果显示一种名为氧化脂质的抗癌蛋白。使用MTT、LDH释放、PI染色、细胞形态分析、溶血和抗增殖试验，对结肠SW480癌细胞系研究了氧化脂质的活性和毒性。氧化脂质是一种从里海眼镜蛇毒液中纯化得到的11kDa蛋白。对所得蛋白的LC/MS/MS分析显示与来自[具体来源未给出]的磷脂酶A2具有同源性。40µg/ml的氧化脂质可在SW480细胞系中诱导高达90%的凋亡效应；与此同时，该剂量对正常细胞仅诱导三分之一的细胞毒性。在本研究中，发现伊朗眼镜蛇毒液对SW480结肠癌细胞具有细胞毒性作用，对HEK - 293正常细胞的细胞毒性最小。鉴于氧化脂质对正常细胞毒性轻微，可以推测所获得的肽在结肠癌动物模型研究中可被视为一种药物先导物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/db8be8aa55ca/ijpr-21-1-129616-i008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/e3f1397f8c5c/ijpr-21-1-129616-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/7a67743bf718/ijpr-21-1-129616-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/7424881ea09a/ijpr-21-1-129616-i003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/bca91c7a903f/ijpr-21-1-129616-i004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/d35bcd81a559/ijpr-21-1-129616-i005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/0f28121f6fe6/ijpr-21-1-129616-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/a3e2ae619f2f/ijpr-21-1-129616-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/a0718d453ab3/ijpr-21-1-129616-i006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/61685a2299bc/ijpr-21-1-129616-i007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/db8be8aa55ca/ijpr-21-1-129616-i008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/e3f1397f8c5c/ijpr-21-1-129616-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/7a67743bf718/ijpr-21-1-129616-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/7424881ea09a/ijpr-21-1-129616-i003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/bca91c7a903f/ijpr-21-1-129616-i004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/d35bcd81a559/ijpr-21-1-129616-i005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/0f28121f6fe6/ijpr-21-1-129616-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/a3e2ae619f2f/ijpr-21-1-129616-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/a0718d453ab3/ijpr-21-1-129616-i006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/61685a2299bc/ijpr-21-1-129616-i007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f72/10016118/db8be8aa55ca/ijpr-21-1-129616-i008.jpg

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本文引用的文献

1
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2
Trends in peptide drug discovery.肽类药物研发趋势。
Nat Rev Drug Discov. 2021 Apr;20(4):309-325. doi: 10.1038/s41573-020-00135-8. Epub 2021 Feb 3.
3
Snake venom three-finger toxins and their potential in drug development targeting cardiovascular diseases.蛇毒三指毒素及其在心脑血管疾病药物开发中的潜力。
Biochem Pharmacol. 2020 Nov;181:114105. doi: 10.1016/j.bcp.2020.114105. Epub 2020 Jun 21.
4
The Influence of the Different Disposition Characteristics of Snake Toxins on the Pharmacokinetics of Snake Venom.蛇毒不同赋存特征对其药代动力学的影响。
Toxins (Basel). 2020 Mar 16;12(3):188. doi: 10.3390/toxins12030188.
5
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6
The first report on coagulation and phospholipase A2 activities of Persian Gulf lionfish, Pterois russelli, an Iranian venomous fish.关于伊朗有毒鱼类波斯湾狮子鱼（细纹狮子鱼）凝血和磷脂酶A2活性的首次报告。
Toxicon. 2016 Apr;113:25-31. doi: 10.1016/j.toxicon.2016.02.004. Epub 2016 Feb 4.
7
Lebein, a snake venom disintegrin, suppresses human colon cancer cells proliferation and tumor-induced angiogenesis through cell cycle arrest, apoptosis induction and inhibition of VEGF expression.勒贝因（Lebein）是一种蛇毒去整合素，它通过细胞周期阻滞、诱导凋亡以及抑制血管内皮生长因子（VEGF）表达来抑制人结肠癌细胞的增殖和肿瘤诱导的血管生成。
Mol Carcinog. 2017 Jan;56(1):18-35. doi: 10.1002/mc.22470. Epub 2016 Jan 29.
8
Cobra venom cytotoxins; apoptotic or necrotic agents?眼镜蛇毒细胞毒素：凋亡剂还是坏死剂？
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9
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