SlMYB72 affects pollen development by regulating autophagy in tomato.

The formation and development of pollen are among the most critical processes for reproduction and genetic diversity in the life cycle of flowering plants. The present study found that was highly expressed in the pollen and tapetum of tomato flowers. Downregulation of led to a decrease in the amounts of seeds due to abnormal pollen development compared with wild-type plants. Downregulation of delayed tapetum degradation and inhibited autophagy in tomato anther. Overexpression of led to abnormal pollen development and delayed tapetum degradation. Expression levels of some autophagy-related genes (ATGs) were decreased in downregulated plants and increased in overexpression plants. SlMYB72 was directly bound to ACCAAC/ACCAAA motif of the promoter and activated its expression. Downregulation of inhibited the autophagy process and tapetum degradation, resulting in abnormal pollen development in tomatoes. These results indicated SlMYB72 affects the tapetum degradation and pollen development by transcriptional activation of and autophagy in tomato anther. The study expands the understanding of the regulation of autophagy by SlMYB72, uncovers the critical role that autophagy plays in pollen development, and provides potential candidate genes for the production of male-sterility in plants.