Muñoz Pilar, Castillejo Cristina, Gómez José Antonio, Miranda Luis, Lesemann Silke, Olbricht Klaus, Petit Aurélie, Chartier Philippe, Haugeneder Annika, Trinkl Johanna, Mazzoni Luca, Masny Agnieszka, Zurawicz Edward, Ziegler Freya Maria Rosemarie, Usadel Björn, Schwab Wilfried, Denoyes Béatrice, Mezzetti Bruno, Osorio Sonia, Sánchez-Sevilla José F, Amaya Iraida
Centro IFAPA de Málaga, Instituto Andaluz de Investigación y Formación Agraria y Pesquera (IFAPA), 29140, Málaga, Spain.
PhD program in Advanced Biotechnology, Universidad de Málaga, 29071, Málaga, Spain.
Hortic Res. 2023 Jan 19;10(3):uhad006. doi: 10.1093/hr/uhad006. eCollection 2023 Mar.
Strawberry ( × ) fruits are an excellent source of -ascorbic acid (AsA), a powerful antioxidant for plants and humans. Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality. Here, we unravel the genetic architecture of AsA accumulation using an F population derived from parental lines 'Candonga' and 'Senga Sengana', adapted to distinct Southern and Northern European areas. To account for environmental effects, the F and parental lines were grown and phenotyped in five locations across Europe (France, Germany, Italy, Poland and Spain). Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold, with significant differences among genotypes and environments. AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus (QTL) analysis. Environmentally stable QTLs for AsA content were detected in linkage group (LG) 3A, LG 5A, LG 5B, LG 6B and LG 7C. Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that and the chloroplast-located AsA transporter gene might be the underlying genetic factors for QTLs on LG 3A and 7C, respectively. We show that recessive alleles of inherited from both parental lines increase fruit AsA content. Furthermore, expression of was two-fold higher in lines with high AsA. Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content.
草莓（×）果实是抗坏血酸（AsA）的优质来源，AsA是一种对植物和人类都有强大抗氧化作用的物质。确定AsA积累的遗传成分对于提高草莓的营养品质至关重要。在这里，我们利用源自亲本‘坎东加’和‘森加·森加纳’的F群体解析了AsA积累的遗传结构，这两个亲本分别适应欧洲南部和北部不同地区。为了考虑环境影响，F群体和亲本在欧洲的五个地点（法国、德国、意大利、波兰和西班牙）种植并进行表型分析。果实AsA含量呈现出数量性状典型的正态分布，范围相差五倍，基因型和环境之间存在显著差异。将每个国家的AsA含量以及所有国家的平均值与6974个标记结合起来用于数量性状位点（QTL）分析。在连锁群（LG）3A、LG 5A、LG 5B、LG 6B和LG 7C中检测到了AsA含量的环境稳定QTL。在稳定的QTL区间内鉴定出了候选基因，对AsA含量不同的品系进行表达分析表明， 和叶绿体定位的AsA转运蛋白基因 可能分别是LG 3A和7C上QTL的潜在遗传因素。我们发现，从两个亲本系遗传而来的 的隐性等位基因会增加果实AsA含量。此外， 在AsA含量高的品系中的表达量高出两倍。这里鉴定出的标记为高效选择AsA含量增加的新草莓品种提供了有用的资源。
