Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Vet Dermatol. 2023 Aug;34(4):348-354. doi: 10.1111/vde.13158. Epub 2023 Mar 20.
Glycerinated allergen extracts contain 50% glycerin, an excellent preservative. While glycerin is a recognised irritant in humans, the utility of glycerinated extracts for intradermal testing has not been validated in dogs.
HYPOTHESIS/OBJECTIVE: To determine and compare the effects of glycerin on immediate cutaneous reactions to intradermal injections of histamine and saline in healthy dogs.
Eight healthy laboratory beagles.
The study was designed as a randomised, blinded study. Intradermal injections of histamine (positive control) and saline (negative control) in aqueous and glycerinated (50%) forms were performed on the right thorax. Global wheal scores (GWS) at 20 min were evaluated by two independent investigators blinded to the interventions.
There were no wheal and flare reactions observed after the intradermal injections of phenolated saline. By contrast, 50% glycerosaline injections induced erythema and induration in all dogs. Global wheal scores were significantly higher in aqueous histamine (Friedman test, p < 0.0001) and 50% glycerinated histamine (Friedman test, p = 0.0084) compared to phenolated saline controls. Interestingly, only aqueous histamine (Friedman test, p = 0.01) had significantly higher GWS than 50% glycerosaline injections, while no significant difference in GWS between 50% glycerinated histamine and 50% glycerosaline groups was observed (Friedman test, p = 0.59).
This study demonstrates that intradermal injection of 50% glycerosaline induces erythema and induration skin reactions in healthy dogs that can mimic positive reactions to allergenic extracts. Further dilutions of glycerinated positive and negative control solutions need to be optimised for intradermal testing in dogs.
甘油化过敏原提取物含有 50%的甘油，这是一种极好的防腐剂。虽然甘油在人类中是一种公认的刺激性物质，但甘油化提取物在犬类皮内试验中的应用尚未得到验证。
假设/目的：确定并比较甘油对健康犬皮内注射组织胺和生理盐水即刻皮肤反应的影响。
8 只健康的实验室比格犬。
该研究设计为随机、双盲研究。在右胸进行组织胺（阳性对照）和生理盐水（阴性对照）的皮内注射，分别为水溶液和 50%甘油化形式。由两名对干预措施不知情的独立研究者在 20 分钟时评估总体风团评分（GWS）。
酚化生理盐水皮内注射后未见风团和红斑反应。相比之下，所有犬只均出现 50%甘油盐水注射后的红斑和硬结。与酚化生理盐水对照相比，水溶液组织胺（Friedman 检验，p<0.0001）和 50%甘油化组织胺（Friedman 检验，p=0.0084）的 GWS 显著更高。有趣的是，只有水溶液组织胺（Friedman 检验，p=0.01）的 GWS 显著高于 50%甘油盐水组，而 50%甘油化组织胺和 50%甘油盐水组之间的 GWS 无显著差异（Friedman 检验，p=0.59）。
本研究表明，50%甘油盐水皮内注射可引起健康犬的红斑和硬结皮肤反应，可模拟对变应原提取物的阳性反应。需要进一步优化甘油化阳性和阴性对照溶液的稀释度，以用于犬类皮内试验。
