Department of Chemistry, Indian Institute of Technology (Banaras Hindu University), Varanasi-221005, India.
Model Rural Health Research Unit, Datia; Indian Council of Medical Research-National Institute of Research in Tribal Health (ICMR-NIRTH), Jabalpur-482003, India.
J Mater Chem B. 2023 Mar 30;11(13):3014-3026. doi: 10.1039/d2tb02256g.
Owing to the rise in multidrug-resistant bacterial diseases and the dwindling supply of newer antibiotics, it is crucial to discover newer compounds or modify current compounds for more effective antimicrobial therapies. According to reports, more than 80% of bacterial infections have been linked to bacterial biofilms. In addition to having antimicrobial properties, the hydrophobic polyphenol curcumin (Cur) also inhibits quorum sensing. The application of curcumin was constrained by its weak aqueous solubility and quick degradation. Over the past years, nanotechnology-based biomaterials with multi-functional characteristics have been engineered with high interest. The present study focused on the development of nano-biomaterials with excellent testifiers for bacterial infection . In this study, water dispersibility and stability of curcumin were improved through conjugation with gold nanoparticles. The successful synthesis of curcumin-conjugated gold nanoparticles (Cur-AuNPs) was confirmed using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and UV-vis absorbance spectroscopy. Transmission electron microscopy (TEM) revealed an average particle size of about 10-13 nm. The antibacterial characteristics in terms of the minimum inhibitory concentration (MIC) of Cur-AuNP treatments were found to be lowest than those with AuNPs and Cur treatments. The quantitative analysis revealed the superior antibacterial characteristics of Cur-AuNP-treated bacterial cells compared to the untreated samples. In addition, curcumin-conjugated AuNPs, produced more reactive oxygen species and increased the membrane permeability. Besides, the biocompatibility of Cur-AuNPs was also assessed quantitatively and qualitatively. Statistical analyses revealed the augmented MG-63 cell proliferation in Cur-AuNPs compared to those with Cur and AuNPs treatments. Overall, Cur-AuNPs exhibited enhanced antibacterial, and antibiofilm characteristics and cytocompatibility.
由于多药耐药菌疾病的增加和新型抗生素供应的减少，发现新的化合物或修饰现有的化合物以进行更有效的抗菌治疗至关重要。据报道，超过 80%的细菌感染与细菌生物膜有关。除了具有抗菌特性外，疏水性多酚姜黄素 (Cur) 还抑制群体感应。由于其弱的水溶解度和快速降解，姜黄素的应用受到限制。在过去的几年中，具有多功能特性的基于纳米技术的生物材料已经引起了极大的关注。本研究专注于开发具有出色抗菌感染测试能力的纳米生物材料。在这项研究中，通过与金纳米粒子缀合来提高姜黄素的水分散性和稳定性。使用 X 射线衍射 (XRD)、傅里叶变换红外 (FTIR) 光谱和紫外可见吸收光谱证实了姜黄素-金纳米粒子 (Cur-AuNPs) 的成功合成。透射电子显微镜 (TEM) 显示平均粒径约为 10-13nm。Cur-AuNP 处理的最低抑菌浓度 (MIC) 的抗菌特性发现比 AuNPs 和 Cur 处理的最低抑菌浓度 (MIC) 更低。定量分析表明，与未经处理的样品相比，Cur-AuNP 处理的细菌细胞具有更好的抗菌特性。此外，姜黄素-金纳米粒子产生更多的活性氧并增加膜通透性。此外，还定量和定性评估了 Cur-AuNPs 的生物相容性。统计分析表明，与 Cur 和 AuNPs 处理相比，Cur-AuNPs 中 MG-63 细胞的增殖增加。总体而言，Cur-AuNPs 表现出增强的抗菌、抗生物膜特性和细胞相容性。
