Department of Medical Physics, Lorestan University of Medical Sciences, Khorramabad, Iran.
Radiation Biology Research Center, Iran University of Medical Sciences (IUMS), 14003391769, Tehran, Iran.
Lasers Med Sci. 2022 Mar;37(2):1333-1341. doi: 10.1007/s10103-021-03399-7. Epub 2021 Aug 18.
Nanoparticle-mediated hyperthermia is one of the prominent adjuvant therapies which has been faced by many problematic challenges such as efficiency and safety. To compare the nanoparticle-mediated photothermal therapy and radiofrequency electric field hyperthermia, green-synthesized curcumin-coated gold nanoparticles (Cur@AuNPs) were applied in an in vitro study. Using recently published methodologies, each step of the study was performed. Through green chemistry, curcumin was applied as both a reducing and a capping agent in the gold nanoparticle synthesis process. Various techniques were applied for the characterization of the synthesized nanoparticles. The heating rate of Cur@AuNPs in the presence of RFEF or laser irradiation was recorded by using a non-contact thermometer. The cellular uptake of the Cur@AuNPs was studied by ICP-AES. The cellular viability and apoptosis rate of different treatment were measured to investigate the effect of two different nano-hyperthermia techniques on the murine colorectal cancer cell line. The average size of Cur@AuNPs was 7.2 ± 3.3 nm. The stability of the gold nanoparticles in the phosphate buffer saline with and without fetal bovine serum was verified by UV-Vis spectroscopy. FTIR, UV-Vis spectroscopy, and TEM indicate that the stability is a result of phenolic coating on the surface of nanoparticles. Cur@AuNPs can absorb both light and radiofrequency electric field exposure in a way that could kill cancerous cells in a significant number (30% in 64 μg/ml concentration). Green-synthesized Cur@AuNPs could induce apoptosis cell death in photothermal therapy and radiofrequency electric field hyperthermia.
纳米粒子介导的热疗是一种突出的辅助治疗方法，但其面临着许多问题和挑战，如效率和安全性。为了比较纳米粒子介导的光热疗法和射频电场热疗，本研究采用绿色合成的姜黄素包覆的金纳米粒子（Cur@AuNPs）进行了体外研究。本研究按照最近发表的方法进行了每一步。通过绿色化学，姜黄素在金纳米粒子的合成过程中既作为还原剂又作为稳定剂。采用各种技术对合成的纳米粒子进行了表征。在存在 RFEF 或激光辐射的情况下，通过使用非接触式温度计记录了 Cur@AuNPs 的升温速率。通过电感耦合等离子体原子发射光谱法（ICP-AES）研究了 Cur@AuNPs 的细胞摄取。测量不同处理的细胞活力和细胞凋亡率，以研究两种不同的纳米热疗技术对鼠结直肠癌细胞系的影响。Cur@AuNPs 的平均粒径为 7.2±3.3nm。通过紫外-可见光谱验证了金纳米粒子在磷酸盐缓冲盐水（含或不含胎牛血清）中的稳定性。傅里叶变换红外光谱（FTIR）、紫外-可见光谱和透射电子显微镜（TEM）表明，这种稳定性是由于纳米粒子表面的酚类涂层。Cur@AuNPs 可以吸收光和射频电场辐射，从而可以杀死大量癌细胞（在 64μg/ml 浓度下为 30%）。绿色合成的 Cur@AuNPs 可以在光热疗法和射频电场热疗中诱导细胞凋亡。
