2nd Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, Hippokration General Hospital, Thessaloniki, Greece.
Laboratory of Gynecologic Oncology, 2nd Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, Hippokration General Hospital, Thessaloniki, Greece.
Diagn Cytopathol. 2023 Jul;51(7):414-422. doi: 10.1002/dc.25130. Epub 2023 Mar 20.
Human papilloma virus (HPV), in addition to its known clinical contribution to cervical cancer is probably actively involved in the development of breast tumors in various populations worldwide. Predominant HPV types in breast cancer patients vary geographically. The present study further examines HPV incidence in Greece, based on molecular analysis of clinical cytological samples.
Greek patient fine needle aspiration (FNA) biopsy samples were examined using RT-PCR and immunohistological staining. FNA biopsy samples were collected from 114 female patients, diagnosed between the years 2018 and 2021, 57 with C5 diagnosed breast cancer lesions and 57 diagnosed with benign diseases.
A total of three different HPV types were identified within the patient sample. HPV-39 was found only in the control group, in 1.8% of patients, while HPV-59 was present in both control and study groups in 1.8% and 3.5% respectively. HPV-16, on the other hand, was present only in the study group in 12.3% of cases. HPV type presence was statistically differentiated between histological groups. HPV-16 was exclusively in IDC, HPV-39 was present in one cyst diagnosed sample and HPV-59 was present in 3 samples that included fibroadenoma, IDC and LN diagnosis.
More international comparative studies are required to investigate population differences and HPV genotype distribution to offer definite answers to the effect that certain HPV types might have a role in breast cancer, as this study also supports, albeit in a cofactory role.
人乳头瘤病毒（HPV）除了已知的对宫颈癌的临床贡献外，可能还积极参与了世界各地不同人群乳腺癌的发生。乳腺癌患者中优势 HPV 型在地理上有所不同。本研究进一步通过对临床细胞学样本的分子分析，在希腊检查 HPV 的发病率。
使用 RT-PCR 和免疫组织化学染色检查希腊患者细针抽吸（FNA）活检样本。FNA 活检样本采集自 114 名女性患者，诊断时间为 2018 年至 2021 年，57 名患有 C5 型乳腺癌病变，57 名患有良性疾病。
在患者样本中总共鉴定出三种不同的 HPV 类型。HPV-39 仅在对照组中发现，占患者的 1.8%，而 HPV-59 则分别在对照组和研究组中占 1.8%和 3.5%。另一方面，HPV-16 仅在研究组的 12.3%的病例中存在。HPV 类型的存在在组织学组之间存在统计学差异。HPV-16 仅存在于 IDC 中，HPV-39 存在于一个囊性诊断样本中，HPV-59 存在于包括纤维腺瘤、IDC 和 LN 诊断的 3 个样本中。
需要进行更多的国际比较研究，以调查人群差异和 HPV 基因型分布，为某些 HPV 类型可能在乳腺癌中发挥作用这一效应提供明确答案，正如本研究也支持的那样，尽管是在共因子作用下。
