Student Research Committee.
Medicinal Plant Research Center.
Behav Pharmacol. 2023 Dec 1;34(8):449-456. doi: 10.1097/FBP.0000000000000721. Epub 2023 Mar 20.
Berberine is an isoquinoline alkaloid naturally produced by several types of plants. Berberine has extensive pharmacological effects, such as anti-diabetic, anti-inflammatory, and antioxidant effects. In the current study, we assess the antinociceptive effects of berberine and its association with the l -arginine ( l -Arg)/NO/cGMP/K ATP channel pathway via intraplantar administration in rats. To examine the antinociceptive properties of berberine, the formalin test was conducted. The number of rat paw flinches was counted for an h. l -Arg (precursor of nitric oxide, 3-30 μ g/paw), l -NAME (NO synthase inhibitor, 10 and 100 μ g/paw), methylene blue (guanylyl cyclase inhibitor, 100 and 200 μ g/paw), and glibenclamide (ATP-sensitive potassium channel blocker, 10 and 30 μ g/paw) were locally injected, respectively, into the right hind paws of rats as a pre-treatment before berberine injection to understand how the l -Arg/NO/cGMP/K ATP pathway plays a role in the antinociceptive effect of berberine. The ipsilateral injection of berberine into the right paw (0.1-10 0 μ g/paw) showed a dose-dependent antinociceptive effect in both the first and second phases of the formalin test, almost similar to morphine (25 μ g/paw). Intraplantar injection of l -Arg (30 µg/paw) increased the antinociceptive effect of berberine in the second phase. In addition, injection of l -NAME, methylene blue, and glibenclamide caused a reduction in the antinociceptive effect of berberine throughout the second phase in a dose-dependent manner. However, the antinociceptive effects of berberine in the first phase of the rat formalin test were not affected by this pathway. As a novel local antinociceptive agent, berberine can exert a peripheral antinociceptive effect via the l -Arg/NO/cGMP/K ATP channel pathway.
小檗碱是一种异喹啉生物碱，天然存在于多种植物中。小檗碱具有广泛的药理作用，如抗糖尿病、抗炎和抗氧化作用。在目前的研究中，我们通过向大鼠足底给药来评估小檗碱的镇痛作用及其与 l -精氨酸（l -Arg）/NO/cGMP/K ATP 通道途径的关系。为了研究小檗碱的镇痛特性，进行了福尔马林试验。在 h 内计数大鼠足底抽搐的次数。l -Arg（一氧化氮的前体，3-30 μ g/爪）、l -NAME（一氧化氮合酶抑制剂，10 和 100 μ g/爪）、亚甲蓝（鸟苷酸环化酶抑制剂，100 和 200 μ g/爪）和格列本脲（ATP 敏感性钾通道阻滞剂，10 和 30 μ g/爪）分别局部注射到大鼠右后爪中，作为小檗碱注射前的预处理，以了解 l -Arg/NO/cGMP/K ATP 途径在小檗碱镇痛作用中的作用。向右侧足底注射小檗碱（0.1-1000 μ g/爪）呈剂量依赖性镇痛作用，在福尔马林试验的第一和第二阶段，几乎与吗啡（25 μ g/爪）相似。足底注射 l -Arg（30 μ g/爪）增加了小檗碱在第二阶段的镇痛作用。此外，以剂量依赖性方式注射 l -NAME、亚甲蓝和格列本脲会降低小檗碱在整个第二阶段的镇痛作用。然而，小檗碱在大鼠福尔马林试验第一阶段的镇痛作用不受该途径的影响。作为一种新型的局部镇痛剂，小檗碱可以通过 l -Arg/NO/cGMP/K ATP 通道途径发挥外周镇痛作用。
