Raziani Yosra, Cheraghipour Koroush, Shakibaie Mojtaba, Ghasemian Yadegari Javad, Mahmoudvand Hossein
Nursing Department, Al-Mustaqbal University College, 51001 Hillah, Babylon, Iraq.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Biomed Pharmacother. 2023 May;161:114536. doi: 10.1016/j.biopha.2023.114536. Epub 2023 Mar 20.
This study examined the synthesis of magnetic iron oxide nanoparticles coated with PO (FOMNPsP) and assessed their in vitro, ex vivo, and in vivo effects against cystic echinococcosis. The FOMNPsP was synthesized through the alkalization of iron ions in a deoxygenated form. In vitro and ex vivo protoscolicidal effects of FOMNPsP (100-400 μg/mL) were evaluated on hydatid cyst protoscoleces by the eosin exclusion test for 10-60 min. The effect of FOMNPsP on caspase-3 gene expression and exterior ultra-structural of protoscoleces was assessed by real-time PCR and scanning electron microscopy (SEM), respectively. In vivo effects were assessed by evaluating the number, size, and weight of hydatid cysts among infected mice. The FOMNPsP size was < 55 nm, and the most frequent particles were in the 15-20 nm range. In vitro and ex vivo assays revealed that the highest protoscolicidal effect was observed at 400 µg/mL with 100% lethality. After exposure of protoscoleces with FOMNPsP, the level of gene expression of caspase-3 was dose-dependently increased (p < 0.05). By SEM, the FOMNPsP-treated protoscoleces showed wrinkles and bulges resulting from the formation of blebs. FOMNPsP significantly decreased (p < 0.01) the mean number, size, and weight of the hydatid cyst. FOMNPsP revealed the potent protoscolicidal traits through disrupting the cell wall and apoptosis induction. The results also indicated the promising effect of FOMNPsP in controlling hydatid cysts in the animal model. Although FOMNPsP is safe for human normal cells, more investigations are required to clarify its toxicity and precise mechanisms of action.
本研究检测了磷酸包覆磁性氧化铁纳米颗粒（FOMNPsP）的合成，并评估了其对囊性棘球蚴病的体外、离体及体内效应。FOMNPsP通过对脱氧形式的铁离子进行碱化反应合成。采用伊红排斥试验，在100 - 400μg/mL浓度下，对包虫囊肿原头节进行10 - 60分钟处理，评估FOMNPsP的体外和离体杀原头节效果。分别通过实时聚合酶链反应和扫描电子显微镜（SEM）评估FOMNPsP对原头节半胱天冬酶 - 3基因表达及外部超微结构的影响。通过评估感染小鼠体内包虫囊肿的数量、大小和重量来评估体内效应。FOMNPsP的粒径小于55nm，最常见的颗粒粒径在15 - 20nm范围内。体外和离体试验表明，在400μg/mL时观察到最高的杀原头节效果，致死率达100%。用FOMNPsP处理原头节后，半胱天冬酶 - 3的基因表达水平呈剂量依赖性增加（p < 0.05）。通过扫描电子显微镜观察，经FOMNPsP处理的原头节出现水泡形成导致的皱纹和凸起。FOMNPsP显著降低了（p < 0.01）包虫囊肿的平均数量、大小和重量。FOMNPsP通过破坏细胞壁和诱导细胞凋亡显示出强大的杀原头节特性。结果还表明FOMNPsP在动物模型中控制包虫囊肿具有良好效果。尽管FOMNPsP对人类正常细胞是安全的，但仍需要更多研究来阐明其毒性和确切作用机制。
