Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Research Center for Hydatid Diseases in Iran, Kerman University of Medical Sciences, Kerman, Iran.
Infect Disord Drug Targets. 2020;20(5):708-712. doi: 10.2174/1871526519666190924160925.
In medicine, ozone therapy is effectively used in a broad spectrum of diseases. Reviews have shown that ozone gas demonstrates potent antimicrobial effects against a wide range of pathogenic microorganisms, such as oral bacteria, fungi, viruses, and parasite even in resistant strains. The present investigation was designed to assess the protoscolicidal effects of ozone gas on hydatid cysts protoscoleces in vitro and in vivo.
Hydatid cyst protoscoleces were acquired from sheep livers that were slaughtered at Kerman slaughterhouse, Iran. The viability of protoscoleces was assessed by the eosin exclusion examination after exposure with ozone gas for 1 to 14 min in vitro and ex vivo.
In this study, in vitro assay showed that ozone gas at the concentration of 20 mg/L killed 85 and 100% of hydatid cyst protoscoleces after 4 and 6 min of treatment, respectively. However, in the ex vivo analysis, a longer time was needed to confirm a potent protoscolicidal activity such that ozone gas after an exposure time of 12 min, 100% of the protoscoleces were killed within the hydatid cyst.
In conclusion, the findings of the present study showed that ozone gas at low concentrations (20 mg/L) and short times (4-6 min) might be used as a novel protoscolicidal drug for use in hydatid cyst surgery. However, more clinical surveys are required to discover the precise biological activity of ozone gas in animal and human subjects.
在医学领域，臭氧疗法被广泛应用于多种疾病。研究表明，臭氧气体对多种致病微生物（如口腔细菌、真菌、病毒和寄生虫）具有强大的抗菌作用，甚至对耐药菌株也有作用。本研究旨在评估臭氧气体对体外和体内包虫囊肿原头蚴的杀原头蚴作用。
从伊朗克尔曼屠宰场屠宰的绵羊肝脏中获得包虫囊肿原头蚴。通过体外和离体试验，用臭氧气体处理 1 至 14 分钟后，用伊红排斥试验评估原头蚴的活力。
本研究显示，体外试验中，浓度为 20mg/L 的臭氧气体分别在 4 分钟和 6 分钟后杀死了 85%和 100%的包虫囊肿原头蚴。然而，在离体分析中，需要更长的时间才能确认强烈的杀原头蚴活性，即臭氧气体暴露 12 分钟后，100%的原头蚴在包虫囊肿内被杀灭。
总之，本研究结果表明，低浓度（20mg/L）和短时间（4-6 分钟）的臭氧气体可作为一种新型的杀原头蚴药物用于包虫囊肿手术。然而，还需要更多的临床研究来发现臭氧气体在动物和人体中的精确生物学活性。
