CALAGUA - Unidad Mixta UV-UPV, Departament d'Enginyeria Química, Universitat de València, Avinguda de la Universitat s/n, 46100 Burjassot, Valencia, Spain.
Department of Chemical Engineering, Søltofts Plads 228A, Technical University of Denmark, DTU, 2800 Lyngby, Denmark.
Bioresour Technol. 2023 May;376:128922. doi: 10.1016/j.biortech.2023.128922. Epub 2023 Mar 20.
Three inhibitors targeting different microorganisms, both from Archaea and Bacteria domains, were evaluated for their effect on CO biomethanation: sodium ionophore III (ETH2120), carbon monoxide (CO), and sodium 2-bromoethanesulfonate (BES). This study examines how these compounds affect the anaerobic digestion microbiome in a biogas upgrading process. While archaea were observed in all experiments, methane was produced only when adding ETH2120 or CO, not when adding BES, suggesting archaea were in an inactivated state. Methane was produced mainly via methylotrophic methanogenesis from methylamines. Acetate was produced at all conditions, but a slight reduction on acetate production (along with an enhancement on CH production) was observed when applying 20 kPa of CO. Effects on CO biomethanation were difficult to observe since the inoculum used was from a real biogas upgrading reactor, being this a complex environmental sample. Nevertheless, it must be mentioned that all compounds had effects on the microbial community composition.
三种针对不同微生物的抑制剂，分别来自古菌域和细菌域，被评估用于其对 CO 生物甲烷化的影响：钠离子载体 III（ETH2120）、一氧化碳（CO）和 2-溴乙磺酸钠（BES）。本研究考察了这些化合物在沼气升级过程中如何影响厌氧消化微生物组。虽然在所有实验中都观察到了古菌，但只有添加 ETH2120 或 CO 时才会产生甲烷，而添加 BES 时则不会，这表明古菌处于失活状态。甲烷主要通过甲基胺的甲基营养型产甲烷生成。在所有条件下都产生了乙酸，但当施加 20 kPa 的 CO 时，观察到乙酸产量略有减少（同时 CH 产量增加）。由于所用的接种物来自实际的沼气升级反应器，这是一个复杂的环境样本，因此难以观察到 CO 生物甲烷化的影响。然而，必须指出的是，所有化合物都对微生物群落组成产生了影响。
