白藜芦醇对乳腺癌和肝癌细胞系的疗效。
Efficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines.
机构信息
From the Department of Home Eonomy, College of Education, Prince Sattam bin Abdulaziz University, Al-kharj, Kingdom of Saudi Arabia.
出版信息
Saudi Med J. 2023 Mar;44(3):246-252. doi: 10.15537/smj.2023.44.3.20220768.
OBJECTIVES
To evaluate the anti-cancer effect of resveratrol on Michigan cancer foundation-7 (MCF-7) and hepatoblastoma cell line (HepG2) cells.
METHODS
The study was carried out at the Department of Botany and Microbiology, Prince Sattam bin Abdulaziz University, Al-kharj, Saudi Arabia, from August 2022 to October 2022. Different concentrations of resveratrol were added to the MCF-7 and HepG2 cell lines. Cell death and proliferation were measured with MTT and Trypan blue exclusion assays. Apoptosis markers were assessed by using a quantitative PCR assay (qPCR).
RESULTS
The resveratrol was shown to suppress the proliferation of MCF-7 and HepG2 cells at dose- and time-dependent. The cytotoxic effect of resveratrol was observed even at 100 μM after 24 hours. In comparison to untreated cells, resveratrol treatment reduced the viability of MCF-7 cells to roughly 57.5% with a half maximal inhibitory concentration (IC) of 51.18 μM and HepG2 cells to 56.2% with an IC of 57.4 μM. Furthermore, in the tested cell lines, resveratrol was able to induce apoptosis mediated by elevated apoptosis markers.
CONCLUSION
Resveratrol appears to be an excellent candidate agent in anticancer therapy in various human cancers.
目的
评估白藜芦醇对密歇根癌症基金会-7（MCF-7）和肝癌细胞系（HepG2）的抗癌作用。
方法
本研究于 2022 年 8 月至 2022 年 10 月在沙特阿拉伯阿尔卡尔吉的沙特阿拉伯王子苏塔姆·本·阿卜杜勒阿齐兹大学植物学和微生物学系进行。向 MCF-7 和 HepG2 细胞系中加入不同浓度的白藜芦醇。使用 MTT 和台盼蓝排除试验测量细胞死亡和增殖。通过实时定量 PCR 检测评估凋亡标志物。
结果
白藜芦醇显示出剂量和时间依赖性抑制 MCF-7 和 HepG2 细胞增殖的作用。即使在 24 小时后浓度达到 100 μM，也观察到白藜芦醇的细胞毒性作用。与未处理的细胞相比，白藜芦醇处理将 MCF-7 细胞的活力降低到约 57.5%，半数最大抑制浓度（IC）为 51.18 μM，将 HepG2 细胞的活力降低到 56.2%，IC 为 57.4 μM。此外，在测试的细胞系中，白藜芦醇能够诱导由升高的凋亡标志物介导的细胞凋亡。
结论
白藜芦醇似乎是各种人类癌症中抗癌治疗的优秀候选药物。
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