基于荧光的化学工具用于监测水溶液和细胞中超声诱导的羟基自由基产生。

Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells.

机构信息

Department of Engineering Science, Parks Road, Oxford, OX1 3PJ, UK.

Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai, Shandong, 264117, P. R. China.

出版信息

Chem Commun (Camb). 2023 Apr 6;59(29):4328-4331. doi: 10.1039/d3cc00364g.

DOI:10.1039/d3cc00364g

PMID:36942986

Abstract

We report the synthesis of hydroxyl-radical (˙OH) responsive fluorescent probes that utilise the 3,5-dihydroxybenzyl (DHB) functionality. 4-Methylumbeliferone-DHB (Umb-DHB) and resorufin-DHB (Res-DHB) in the presence of ˙OH radicals resulted in significant increases in their respective fluorescent emission intensities at 460 nm and 585 nm. The incubation of Res-DHB in HeLa cells followed by therapeutic ultrasound (1 MHz) resulted in a significant increase in fluorescence emission intensity thus permitting the ability to monitor ultrasound-induced ˙OH production in live cells.

摘要

我们报告了利用 3,5-二羟基苄基 (DHB) 官能团合成羟基自由基 (˙OH) 响应性荧光探针。在 ˙OH 自由基存在下，4-甲基伞形酮-DHB (Umb-DHB) 和 Res-DHB 分别导致其各自的荧光发射强度在 460nm 和 585nm 处显著增加。将 Res-DHB 孵育在 HeLa 细胞中，然后进行治疗性超声 (1MHz)，导致荧光发射强度显著增加，从而能够监测活细胞中超声诱导的 ˙OH 产生。