Ogunro Olalekan Bukunmi, Ofeniforo Emmanuel Bankole, Fakayode Aderonke Elizabeth
Department of Biological Sciences, KolaDaisi University, Ibadan, 200213, Nigeria.
Department of Biochemistry, University of Ilorin, Ilorin, 240222, Nigeria.
J Genet Eng Biotechnol. 2023 Mar 21;21(1):36. doi: 10.1186/s43141-023-00489-7.
The prevalence of diarrhoea remains high despite efforts by governments and NGOs to reverse trend. This study investigated the antidiarrhoeal activity and mechanism of Spondias mombin leaf fraction rich in quercetin-3-O-β-D-glucopyranoside (Q3G-RF) because of the acclaimed therapeutic efficacy. Secretory, osmotic, and infectious diarrhoea models using castor oil, magnesium sulphate, and Shigella flexneri respectively were evaluated at the doses of 100, 200, and 400 mg/kg in Wistar rats. Enteropathy was induced with castor oil and magnesium sulphate, while gastrointestinal motility was determined with charcoal meal.
Findings showed no mortality after 14 days of experimental period and no significant changes in behaviour, food, and water consumption. Relative to control, Q3G-RF inhibited the three models of diarrhoea, enteropathy, and gastrointestinal motility; bacterial colonies were reduced by Q3G-RF, while it improved the relative body weight of the animals. Q3G-RF also increased the intestinal concentration/activity of glucose, total protein, and Na-K ATPase but reduced the concentration of TNF-α, PGE, IL-1β, nitric oxide, Na, K, and Cl in the diarrhoeal models. The intestinal fluid level of K, Na, and Cl was significantly decreased by Q3G-RF in the enteropathy model. Length of the small intestine in the motility model was also increased by Q3G-RF, while peristaltic index and inhibition of peristalsis were reduced.
Overall, quercetin-3-O-β-D-glucopyranoside from Spondias mombin leaves demonstrated efficacy against infectious, secretory, and osmotic form of diarrhoeal and further justified its traditional use in the treatment of diarrhoea due to its antimotility, antisecretory, and antimicrobial properties by mechanism related to enhanced Na-K ATPase, repressed nitric oxide, and suppressed prostaglandins.
尽管政府和非政府组织努力扭转腹泻流行趋势，但腹泻的患病率仍然很高。本研究调查了富含槲皮素-3-O-β-D-吡喃葡萄糖苷（Q3G-RF）的腰果树（Spondias mombin）叶提取物的止泻活性及其作用机制，因其具有公认的治疗效果。分别使用蓖麻油、硫酸镁和福氏志贺菌建立分泌性、渗透性和感染性腹泻模型，以100、200和400mg/kg的剂量在Wistar大鼠中进行评估。用蓖麻油和硫酸镁诱导肠病，用炭末测定胃肠动力。
结果显示，实验期14天后无死亡情况，行为、食物和水摄入量无显著变化。相对于对照组，Q3G-RF抑制了三种腹泻模型、肠病和胃肠动力；Q3G-RF减少了细菌菌落数量，同时提高了动物的相对体重。Q3G-RF还提高了腹泻模型中葡萄糖、总蛋白和钠钾ATP酶的肠道浓度/活性，但降低了肿瘤坏死因子-α、前列腺素E、白细胞介素-1β、一氧化氮、钠、钾和氯的浓度。在肠病模型中，Q3G-RF显著降低了钾、钠和氯的肠液水平。在动力模型中，Q3G-RF还增加了小肠长度，同时降低了蠕动指数和蠕动抑制率。
总体而言，腰果树叶中的槲皮素-3-O-β-D-吡喃葡萄糖苷对感染性、分泌性和渗透性腹泻均有疗效，并进一步证明了其在治疗腹泻方面的传统用途，其抗动力、抗分泌和抗菌特性的作用机制与增强钠钾ATP酶、抑制一氧化氮和抑制前列腺素有关。
