College of Resources, Sichuan Agricultural University, Chengdu, 611130, China.
Sichuan Institute of Edible Fungi, Chengdu, 610066, China.
Can J Microbiol. 2023 Jul 1;69(7):262-278. doi: 10.1139/cjm-2022-0189. Epub 2023 Mar 21.
is a widely prized basidiomycetous mushroom with culinary and medicine value, which is cultivated artificially on a large scale in China. However, little attention has been paid to the differences in metabolic profiles under different pH growth conditions. In the present study, weakly acidic and weakly alkaline artificial substrates were developed and used for the cultivation of , and the metabolic profiles of its fruiting bodies were determined using ultra performance liquid chromatography-tandem mass spectrometry. The results show that the weakly alkaline substrate environment promoted mycelial growth, increased body surface area, and improved yield and transformation efficiency, but attenuated metabolite accumulation by . A total of 412 different metabolites were identified in negative and positive ion mode, of which 99 had significantly different amounts and covered 51 metabolic pathways. Principal component analysis and orthogonal patrial least squares discriminant analysis showed clear separation between two treatments, indicating different metabolic profiles. The different metabolites mainly included seven chemical categories, including amino acids and derivatives, nucleotides and derivatives, phenolic acids, organic acids, lipids, flavonoids and alkaloids. This study revealed the biological significance of these metabolites, which could be useful for further unexplored compounds and possible biological functions of .
是一种广受欢迎的担子菌蘑菇，具有食用和药用价值，在中国已大规模人工种植。然而，人们对不同 pH 值生长条件下的代谢谱差异关注甚少。本研究开发了弱酸性和弱碱性人工基质来栽培 ，并采用超高效液相色谱-串联质谱法测定其子实体的代谢谱。结果表明，碱性基质环境促进了菌丝体的生长，增加了表面积，提高了产量和转化率，但减弱了 的代谢产物积累。在正负离子模式下共鉴定出 412 种不同的代谢物，其中 99 种代谢物的含量有显著差异，涵盖了 51 条代谢途径。主成分分析和正交偏最小二乘判别分析表明，两种处理之间有明显的分离，表明代谢谱不同。不同的代谢物主要包括 7 种化学类别，包括氨基酸及其衍生物、核苷酸及其衍生物、酚酸、有机酸、脂类、类黄酮和生物碱。本研究揭示了这些代谢物的生物学意义，这可能有助于进一步探索 和可能的生物功能。
