Petrović Jovana, Glamočlija Jasmina, Milinčić Danijel D, Doroški Ana, Lević Steva, Stanojević Slađana P, Kostić Aleksandar Ž, Minić Dušanka A Popović, Vidović Bojana B, Plećić Ana, Nedović Viktor A, Pešić Mirjana B, Stojković Dejan
Institute for Biological Research, Siniša Stanković"-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia.
Institute of Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.
Pharmaceuticals (Basel). 2024 Aug 31;17(9):1153. doi: 10.3390/ph17091153.
Herein we describe the antioxidant, antimicrobial, antibiofilm, anti-inflammatory and wound-healing potential of aqueous and polysaccharide extracts from three widely appreciated mushrooms: , and . Moreover, we present their detailed phenolic, polysaccharide and protein profiles and ATR-FTIR spectra. The study found that polysaccharide extracts (PEs) from mushrooms had higher total and -glucan levels than aqueous extracts (AEs), with showing the highest content. had a higher total protein content, and AE had the highest phenolic content. Our results indicate that all the tested extracts have high potential regarding their bioactive properties, with being the most promising one. Namely, the antibacterial activity assay showed that the development of the skin-infection-causing agent, , was inhibited with a minimal inhibitory concentration of 4.00 mg/mL and minimal bactericidal concentration of 8.00 mg/mL, while the results regarding wound healing showed that, over the course of 24 h, the extract actively promoted wound closure in the HaCaT keratinocyte cell line model. The anti-inflammatory activity results clearly showed that when we used as an inflammation-inducing agent and the aqueous extract in treatment, IL-6 levels reduced to the level of 4.56 pg/mL. The obtained data suggest that the tested mushroom extracts may serve as a source of bioactive compounds, with potential applications in the cosmeceutical, pharmaceutical and food industries. Furthermore, potential skin preparations carefully crafted with mushroom extract may help restore the skin's barrier function, decrease the probability of staph infections and minimize skin irritation.
在此，我们描述了三种广受欢迎的蘑菇（[蘑菇名称1]、[蘑菇名称2]和[蘑菇名称3]）的水提取物和多糖提取物的抗氧化、抗菌、抗生物膜、抗炎和伤口愈合潜力。此外，我们还展示了它们详细的酚类、多糖和蛋白质谱以及衰减全反射傅里叶变换红外光谱（ATR-FTIR）。研究发现，蘑菇的多糖提取物（PEs）比水提取物（AEs）具有更高的总β-葡聚糖水平，其中[蘑菇名称1]的含量最高。[蘑菇名称2]的总蛋白质含量较高，而[蘑菇名称3]的水提取物酚类含量最高。我们的结果表明，所有测试提取物在其生物活性特性方面都具有很高的潜力，其中[蘑菇名称1]最具前景。具体而言，抗菌活性测定表明，引起皮肤感染的病原体[病原体名称]的生长受到抑制，最低抑菌浓度为4.00 mg/mL，最低杀菌浓度为8.00 mg/mL，而伤口愈合结果表明，在24小时内，[蘑菇名称1]提取物在HaCaT角质形成细胞系模型中积极促进伤口闭合。抗炎活性结果清楚地表明，当我们使用[炎症诱导剂名称]作为炎症诱导剂并使用[蘑菇名称3]水提取物进行治疗时，白细胞介素-6水平降至4.56 pg/mL。获得的数据表明，测试的蘑菇提取物可能作为生物活性化合物的来源，在药妆、制药和食品工业中具有潜在应用。此外，精心制备的含蘑菇提取物的潜在皮肤制剂可能有助于恢复皮肤屏障功能，降低葡萄球菌感染的可能性，并使皮肤刺激最小化。
