Kemahli Eray, Uyeturk Ugur, Cetinkaya Ayhan, Erimsah Sevilay, Uyeturk Ummugul, Gucuk Adnan
Department of Urology, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey.
Department of Medical Oncology, Istanbul Okan University, Faculty of Medicine, Istanbul, Turkey.
J Coll Physicians Surg Pak. 2023 Mar;33(3):319-324. doi: 10.29271/jcpsp.2023.03.319.
ABSTRACT Objective: To assess the effectiveness of utilising N-acetyl cysteine (NAC) to treat tissue damage brought on by undescended testis (UT) in rats after orchiopexy.
Experimental study. Place and Duration of the Study: Bolu Abant İzzet Baysal University, Bolu, Turkey, from January 2018 to June 2020.
The UT model was created by administering flutamide to pregnant rats. Four groups of animals were created as the control group (offsprings of pregnant rats without flutamide), group II (UT), group III (UT + orchiopexy), and group IV (UT + orchiopexy + NAC); each containing eight animals.
Group IV had a higher level of glutathione peroxidase than groups III and II (p=0.001 and p=0.002, respectively). Malondialdehyde was reduced in group IV compared with groups III and II (both p<0.001). There were differences in mean apoptotic cell counts (ACC) among the groups (p<0.001). ACC in group IV was lower than in group III (p<0.001). Sperm counts were higher in group IV than ın groups III and II, and in group III they were higher than group II (p<0.001 all) and similar between groups IV and control group (p=0.102).
Orchiopexy reduced UT-related testicular damage, additionally using NAC following orchiopexy may further reduce testicular damage through its antioxidant effects.
Undescended testis, Testis damage, Orchiopexy, N-acetyl cysteine, Antioxidant.
摘要 目的：评估在大鼠睾丸固定术后使用N-乙酰半胱氨酸（NAC）治疗隐睾（UT）所致组织损伤的有效性。
实验研究。研究地点和时间：2018年1月至2020年6月，土耳其博卢阿邦特·伊泽特·贝萨尔大学。
通过给孕鼠服用氟他胺建立UT模型。将动物分为四组：对照组（未服用氟他胺的孕鼠后代）、第二组（UT）、第三组（UT + 睾丸固定术）和第四组（UT + 睾丸固定术 + NAC）；每组包含八只动物。
第四组的谷胱甘肽过氧化物酶水平高于第三组和第二组（分别为p = 0.001和p = 0.002）。与第三组和第二组相比，第四组的丙二醛含量降低（均p < 0.001）。各组间平均凋亡细胞计数（ACC）存在差异（p < 0.001）。第四组的ACC低于第三组（p < 0.001）。第四组的精子计数高于第三组和第二组，第三组的精子计数高于第二组（均p < 0.001），第四组与对照组相似（p = 0.102）。
睾丸固定术可减轻与UT相关的睾丸损伤，睾丸固定术后额外使用NAC可能通过其抗氧化作用进一步减轻睾丸损伤。
隐睾；睾丸损伤；睾丸固定术；N-乙酰半胱氨酸；抗氧化剂
