College of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
School of Life Healthy and Technology, Dongguan University of Technology, Dongguan, 523808, China.
Food Funct. 2023 Apr 24;14(8):3552-3563. doi: 10.1039/d2fo03614b.
As a traditional fermented meat product, dry-cured Xuanwei ham could be a rich source of bioactive peptides. This study intended to investigate the transepithelial transport and cytoprotection of antioxidant peptides isolated from simulated gastrointestinal digestion of Xuanwei ham. Through ultrafiltration and gel filtration chromatography after simulated digestion, five new antioxidative cell-penetrating peptides (CPPs) with 16-27 amino acid residues were identified, and protective effects of the pretreatment with GHYTEGAELVDSVLDVVRK (GK-19) and TDEFQLHTNVNDGTEFGGSIYQK (TK-23) on HO-induced damaged HepG2 cells were investigated. The results showed that the peptide TK-23 at 0.5 mg mL showed a good antioxidant activity through upregulating the activity of antioxidant enzymes (CAT, SOD and GR) and decreasing the MDA level in HO-induced damaged HepG2 cells with a better protective effect compared to GSH. Our observations of novel antioxidant CPPs with 16-27 amino acid residues could enrich the antioxidative CPP database, and these findings could provide data support for further study of CPPs.
作为一种传统的发酵肉制品，宣威火腿可能是生物活性肽的丰富来源。本研究旨在探讨从宣威火腿模拟胃肠消化中分离得到的抗氧化肽的跨上皮转运和细胞保护作用。通过模拟消化后的超滤和凝胶过滤层析，鉴定出了 5 种具有 16-27 个氨基酸残基的新型抗氧化细胞穿透肽（CPPs），并研究了 GHYTEGAELVDSVLDVVRK（GK-19）和 TDEFQLHTNVNDGTEFGGSIYQK（TK-23）预处理对 HO 诱导损伤的 HepG2 细胞的保护作用。结果表明，肽 TK-23 在 0.5mg/mL 时通过上调抗氧化酶（CAT、SOD 和 GR）的活性和降低 MDA 水平，对 HO 诱导损伤的 HepG2 细胞表现出良好的抗氧化活性，其保护作用优于 GSH。我们观察到具有 16-27 个氨基酸残基的新型抗氧化 CPP，可以丰富抗氧化 CPP 数据库，这些发现可以为 CPP 的进一步研究提供数据支持。
