School of Life and Health Technology, Dongguan University of Technology, Dongguan 523808, China; College of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China.
School of Life and Health Technology, Dongguan University of Technology, Dongguan 523808, China; Dongguan Prefabricated Food Innovation Development and Quality Control Key Laboratory, Dongguan 523808, China.
Food Chem. 2024 Jul 15;446:138600. doi: 10.1016/j.foodchem.2024.138600. Epub 2024 Feb 1.
An ethanol/(NH)SO biphasic (aqueous two-phase) system was designed to effectively separate antioxidant peptides from Xuanwei ham, and its potential to prevent ultraviolet A-induced damage to skin cells was explored. Optimization via single factor experiments and response surface methodology revealed that under 20 % ethanol aqueous solution (w/w), 25.5 % (NH)SO aqueous solution (w/w), and pH 8.80 conditions, the optimal extraction ratio was 59.0 ± 1.73 %. In vitro antioxidant activity and cellular assays showed that the peptide purified in the upper phase exhibited strong antioxidant activity, increasing the viability of HaCat cells damaged by UVA irradiation from 56.14 ± 1.05 % to 66.3 ± 1.76 %. We used an in silico peptide screening strategy and identified 10 with potential antioxidant activity, emphasizing the important role of amino acids Pro, Gly, and Ala in antioxidant activity.
设计了乙醇/（NH 4 ）2 SO 4 双水相（aqueous two-phase）体系，以有效从宣威火腿中分离抗氧化肽，并探讨其预防 UVA 诱导皮肤细胞损伤的潜力。通过单因素实验和响应面法优化，在 20%乙醇水溶液（w/w）、25.5%（NH 4 ）2 SO 4 水溶液（w/w）和 pH 8.80 条件下，最佳提取率为 59.0±1.73%。体外抗氧化活性和细胞试验表明，在上相纯化的肽具有很强的抗氧化活性，将 UVA 照射损伤的 HaCat 细胞的存活率从 56.14±1.05%提高到 66.3±1.76%。我们使用了一种肽的计算机筛选策略，鉴定出 10 种具有潜在抗氧化活性的肽，强调了氨基酸 Pro、Gly 和 Ala 在抗氧化活性中的重要作用。
