Yin Lichun, Wang Xingshuo, Li Yangyang, Liu Ziyin, Mei Qinyuan, Chen Zeyou
College of Environmental Science and Engineering, Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, Tianjin 300071, China.
J Agric Food Chem. 2023 Mar 22;71(11):4561-4570. doi: 10.1021/acs.jafc.3c01052. Epub 2023 Mar 9.
Antibiotics are used to control certain bacterial diseases in plant agriculture. Understanding antibiotic uptake by edible vegetables after application and associated risks on plant microbiome and human health is critical. In this study, oxytetracycline and streptomycin, the two most commonly used antibiotics in plant agriculture, were applied to cherry radish via continuous soil drenching to study their translocations into plant tissues, influence on radish microbiome, and the potential health risk to mice. The results demonstrated that oxytetracycline induced hormesis in radish plants and both antibiotics were translocated into the leaves, fruits, and roots of radishes from the soil, with significantly higher plant uptake of streptomycin than oxytetracycline. Interestingly, the proportion of culturable oxytetracycline or streptomycin-resistant bacteria in the antibiotic-accumulated radish tissues was significantly higher than that in the antibiotic-free radish tissues, although both bacterial and fungal communities in different radish tissues were not affected by the accumulated antibiotics, demonstrating that antibiotic application could enrich antibiotic resistance in the plant microbiome. Feeding mice with antibiotics-accumulated radish tissues did not show significant effects on the weight and blood glucose levels of mice. Overall, this study provides important insights into the risk of using antibiotics in plant agriculture.
抗生素被用于控制植物农业中的某些细菌性疾病。了解施用后可食用蔬菜对抗生素的吸收以及对植物微生物群和人类健康的相关风险至关重要。在本研究中，通过连续土壤浇灌将植物农业中最常用的两种抗生素土霉素和链霉素施用于樱桃萝卜，以研究它们向植物组织中的转运、对萝卜微生物群的影响以及对小鼠的潜在健康风险。结果表明，土霉素在萝卜植株中诱导了刺激效应，两种抗生素均从土壤转运至萝卜的叶、果实和根中，链霉素在植物中的吸收量显著高于土霉素。有趣的是，在抗生素积累的萝卜组织中，可培养的耐土霉素或耐链霉素细菌的比例显著高于无抗生素的萝卜组织，尽管不同萝卜组织中的细菌和真菌群落均未受到积累抗生素的影响，这表明施用抗生素可使植物微生物群中的抗生素抗性增强。用积累抗生素的萝卜组织喂养小鼠对小鼠的体重和血糖水平未显示出显著影响。总体而言，本研究为植物农业中使用抗生素的风险提供了重要见解。
