Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan, USA.
Faculty of Dentistry, National University of Singapore, Singapore.
J Mater Chem B. 2023 May 3;11(17):3823-3835. doi: 10.1039/d2tb02788g.
Regenerative endodontics represents a paradigm shift in dental pulp therapy for necrotic young permanent teeth. However, there are still challenges associated with attaining maximum root canal disinfection while supporting angiogenesis and preserving resident stem cells viability and differentiation capacity. Here, we developed a hydrogel system by incorporating antibiotic-eluting fiber-based microparticles in gelatin methacryloyl (GelMA) hydrogel to gather antimicrobial and angiogenic properties while prompting minimum cell toxicity. Minocycline (MINO) or clindamycin (CLIN) was introduced into a polymer solution and electrospun into fibers, which were further cryomilled to attain MINO- or CLIN-eluting fibrous microparticles. To obtain hydrogels with multi-therapeutic effects, MINO- or CLIN-eluting microparticles were suspended in GelMA at distinct concentrations. The engineered hydrogels demonstrated antibiotic-dependent swelling and degradability while inhibiting bacterial growth with minimum toxicity in dental-derived stem cells. Notably, compared to MINO, CLIN hydrogels enhanced the formation of capillary-like networks of endothelial cells and the presence of widespread vascularization with functioning blood vessels Our data shed new light onto the clinical potential of antibiotic-eluting gelatin methacryloyl hydrogel as an injectable scaffold with multi-therapeutic effects to promote antimicrobial disinfection and angiogenesis for regenerative endodontics.
再生牙髓学代表着牙髓坏死年轻恒牙治疗的范式转变。然而，在实现最大根管消毒的同时，支持血管生成和保留常驻干细胞的活力和分化能力仍然存在挑战。在这里，我们通过将载抗生素纤维基微球纳入明胶甲基丙烯酰（GelMA）水凝胶中，开发了一种水凝胶系统，以聚集抗菌和血管生成特性，同时促使最小的细胞毒性。米诺环素（MINO）或克林霉素（CLIN）被引入聚合物溶液中并静电纺丝成纤维，然后进一步冷冻研磨以获得 MINO 或 CLIN 载药纤维状微球。为了获得具有多种治疗效果的水凝胶，将 MINO 或 CLIN 载药微球以不同浓度悬浮在 GelMA 中。所设计的水凝胶表现出依赖抗生素的溶胀和降解性，同时抑制细菌生长，对牙源性干细胞的毒性最小。值得注意的是，与 MINO 相比，CLIN 水凝胶增强了内皮细胞毛细血管样网络的形成，并存在广泛的血管化，有功能的血管 我们的数据为载抗生素明胶甲基丙烯酰水凝胶作为一种具有多种治疗效果的可注射支架在再生牙髓学中的临床潜力提供了新的认识，以促进抗菌消毒和血管生成。
