Pardridge W M, Yang J, Eisenberg J, Tourtellotte W W
Department of Medicine, UCLA School of Medicine 90024.
J Neurosci Res. 1987;18(2):352-7. doi: 10.1002/jnr.490180213.
The development of methods for the isolation of brain capillaries and brain capillary plasma membranes makes possible biochemical studies of the blood-brain barrier (BBB), which is made up of brain capillaries. Studies aimed at assessing the role of the BBB in the pathogenesis of specific neurologic diseases, e.g., Alzheimer's disease or multiple sclerosis, will necessitate the isolation of capillaries from brain involved with specific pathology. Such tissue is most readily available from banks containing frozen human brain. The present studies show that intact capillaries and capillary plasma membranes can be isolated from frozen human brain, including as little as five g of multiple sclerosis plaque tissue. Capillaries from frozen human brain are enriched in gamma-glutamyl transpeptidase, factor VIII antigen, and a 46K protein which has recently been shown to be a BBB-specific protein. These studies provide the basis for future biochemical studies of human brain microvessels in neurologic disease.
脑毛细血管及脑毛细血管质膜分离方法的发展，使得对由脑毛细血管构成的血脑屏障（BBB）进行生化研究成为可能。旨在评估血脑屏障在特定神经疾病（如阿尔茨海默病或多发性硬化症）发病机制中作用的研究，将需要从患有特定病理的脑组织中分离毛细血管。此类组织最容易从保存有冷冻人脑的库中获取。目前的研究表明，完整的毛细血管和毛细血管质膜能够从冷冻人脑，包括仅5克的多发性硬化症斑块组织中分离出来。来自冷冻人脑的毛细血管富含γ-谷氨酰转肽酶、因子VIII抗原以及一种46K蛋白，该蛋白最近已被证明是一种血脑屏障特异性蛋白。这些研究为未来对神经疾病中人类脑微血管进行生化研究奠定了基础。
