Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, ON, P7B 5E1, Canada.
Arch Microbiol. 2023 Mar 22;205(4):130. doi: 10.1007/s00203-023-03470-7.
The demand for enzymes is increasing continuously due to their applications in various avenues. The pectin-hydrolyzing bacteria, Cellulomonas sp. and Bacillus sp., isolated from forest soil have the potential to produce industrially important enzymes (pectinase, PGase, Cellulase, and xylanase). However, these bacteria have different optimal cultural conditions for pectinase production. The optimal cultural conditions for Cellulomonas sp. were room temperature (25-26℃), pH 7, 1% inoculum volume, and 1.5% citrus pectin with 8.82 ± 0.92 U/mL pectinase activity. And Bacillus sp. illustrated the highest pectinase activity (12.35 ± 0.72 U/mL) at room temperature, pH 10, 1% inoculum volume, and 1.5% pectin concentration. Among the different agro-wastes, the orange peel was found to be the best substrate for pectinase, PGase, and cellulase activity whereas barley straw for xylanase activity. Further, Cellulomonas sp. and Bacillus sp. illustrated higher pectinase activity from commercial pectin compared to orange peel showing their preference for commercial citrus pectin. In addition, the optimization by the Box-Behnken design increased pectinase activity for Cellulomonas sp., while a noticeable increase in activity was not observed in Bacillus sp. Besides, all the agro-wastes exploited in this study can be used for pectinase, PGase, and xylanase production but not cellulase. The study revealed that each bacteria has its specific optimal conditions and there is a variation in the capacity of utilizing the various lignocellulosic biomass.
由于酶在各个领域的应用，对其的需求正在不断增加。从森林土壤中分离出的果胶水解细菌，纤维单胞菌属和芽孢杆菌属，具有产生工业上重要的酶（果胶酶、PG 酶、纤维素酶和木聚糖酶）的潜力。然而，这些细菌在生产果胶酶方面有不同的最佳培养条件。纤维单胞菌属的最佳培养条件为室温（25-26℃）、pH7、1%接种量和 1.5%柑橘果胶，果胶酶活为 8.82±0.92U/mL。芽孢杆菌属在室温、pH10、1%接种量和 1.5%果胶浓度下表现出最高的果胶酶活性（12.35±0.72U/mL）。在不同的农业废弃物中，橙皮被发现是果胶酶、PG 酶和纤维素酶活性的最佳底物，而大麦秸秆则是木聚糖酶活性的最佳底物。此外，与橙皮相比，纤维单胞菌属和芽孢杆菌属从商业果胶中表现出更高的果胶酶活性，表明它们更喜欢商业柑橘果胶。此外，通过 Box-Behnken 设计进行的优化提高了纤维单胞菌属的果胶酶活性，而芽孢杆菌属的活性没有明显增加。此外，本研究中利用的所有农业废弃物都可用于生产果胶酶、PG 酶和木聚糖酶，但不能用于生产纤维素酶。研究表明，每种细菌都有其特定的最佳条件，并且利用各种木质纤维素生物质的能力存在差异。
