The Smartphone Addiction Scale Short Version (SAS-SV) has been widely used in research, but little is known about the measurement invariance across gender. The current study measured SAS-SV invariance between male and female college students in a sample of 1112 participants. Single- and multiple-group confirmatory factor analyses (CFAs) of smartphone addiction symptom ratings were conducted using R program with RStudio. SAS-SV was psychometrically robust in measuring the severity of smartphone addiction among college students, as well as the gender-based invariance. The differences in SAS-SV between male and female participants were likely to represent true gender differences, and meaningful comparisons could be made.